上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (11): 1328-.

• 论著（基础研究） • 上一篇    下一篇

A3腺苷受体激动剂预处理对兔体外循环中肺组织损伤的影响

鲍春荣, 梅 举, 丁芳宝, 张韫佼   

  1. 上海交通大学 医学院新华医院 心胸外科, 上海 200092
  • 出版日期:2009-11-25 发布日期:2009-11-24
  • 通讯作者: 梅 举, 电子信箱: jumei@yahoo.cn。
  • 作者简介:鲍春荣(1977—), 男, 主治医师, 博士；电子信箱: bcron@126.com。
  • 基金资助:

    上海交通大学医学院新华医院基金（08YJ09）

Effects of A3 adenosine receptor antagonist on lung injury after cardiopulmonary bypass in rabbits

BAO Chun-rong, MEI Ju, DING Fang-bao, ZHANG Yun-jiao   

  1. Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
  • Online:2009-11-25 Published:2009-11-24
  • Supported by:

    Foundation of Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, 08YJ09

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摘要：

目的 研究A3腺苷受体(A3AR)激动剂IB-MECA预处理对兔体外循环(CPB)中肺组织损伤的影响。方法 24只新西兰大白兔随机分为对照组（仅行体外循环）、激动剂组（阻断主动脉前15 min静脉注射IB-MECA）和拮抗剂+激动剂组（IB-MECA干预前15 min静脉注射选择性A3AR拮抗剂MRS-1191），每组8只。CPB结束后采集样本，分别检测和比较各组血清肿瘤坏死因子-α(TNF-α)和白介素-8(IL-8)水平、肺组织丙二醛(MDA)含量和髓过氧化物酶(MPO)活性、肺组织湿/干重比(W/D)、氧合指数(PaO2/FiO2)、肺血管阻力(PVR)和气道压(AWP)等指标，观察各组动物肺组织病理学改变。结果 CPB后，激动剂组血清TNF-α和IL-8水平显著低于对照组和拮抗剂+激动剂组 (P<0.05)；与对照组和拮抗剂+激动剂组比较，激动剂组CPB后肺组织W/D较小，MDA含量较少，MPO活性较低，组间比较差异均有统计学意义（P<0.05)；  CPB后，激动剂组PaO2/FiO2显著高于对照组和拮抗剂+激动剂组(P<0.05)，而AWP和PVR显著低于对照组和拮抗剂+激动剂组(P<0.05)；组织学观察发现，激动剂组肺组织的组织病理学改变较对照组和拮抗剂+激动剂轻微。结论 A3AR激动剂IB-MECA预处理可显著减轻CPB过程中的肺组织损伤程度。

关键词: A3腺苷受体激动剂, 体外循环, 肺损伤

Abstract:

Objective To investigate the effects of A3 adenosine receptor (A3AR) agonist on lung injury after cardiopulmonary bypass (CPB) in rabbits. Methods Twenty-four rabbits were randomly divided into 3 groups, with 8 in each group. Rabbits in control group only received CPB, those in agonist group were given selective A3AR agonist IB-MECA intravenously 15 min before aorta clamp, and those in agonist+antagonist group were managed with selective A3AR receptor antagonist MRS-1191 intravenously before IB-MECA infusion. After CPB, serum concentrations of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8), concentrations of malondialdehyde (MDA) and myeloperoxidase (MPO) in lung tissues, lung wet/dry weight ratio (W/D), lung function related indexes of PaO2/FiO2, airway pressure (AWP) and pulmonary vascular resistance (PVR), and histological changes of lung tissues were observed.ResultsConcentrations of serum TNF-α and IL-8 were significantly lower in agonist group than in control group and agonist+antagonist group (P<0.05). Compared with control group and agonist+antagonist group, W/D was much smaller, and concentrations of MDA and MPO were significantly lower in agonist group after CPB （P<0.05). PaO2/FiO2 was significantly higher, while AWP and PVR were significantly lower in agonist group than in control group and agonist+antagonist group (P<0.05). It was revealed by histological examinations that the pathological changes were less severe in agonist group than in control group and agonist+antagonist group. Conclusion A3AR agonist IB-MECA can reduce lung injury after CPB.

Key words: A3 adenosine receptor agonist, cardiopulmonary bypass, lung injury