上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (4): 436-.

• 论著（临床研究） • 上一篇    下一篇

炎症积水的输卵管上皮组织中Toll样受体及信号转导通路的表达

陈淑芳, 陈 忆, 李玉宏, 奚 吉, 张 健   

  1. 上海交通大学 医学院国际和平妇幼保健院, 上海 200030
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 通讯作者: 张 健, 电子信箱: zjian65@hotmail.com。
  • 作者简介:陈淑芳(1973—), 女, 主治医师, 硕士生；电子信箱: csf_bjy@yahoo.com.cn。
  • 基金资助:

    上海交通大学医学院基金(06XJ21118)；上海市卫生局基金(2007164)；上海市自然科学基金（08ZR1421200）

Expression of Toll-like receptor and signal transduction pathway in tubal epithelial tissues with inflammatory hydrosalpinx

CHEN Shu-fang, CHEN Yi, LI Yu-hong, XI Ji, ZHANG Jian   

  1. International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China
  • Online:2010-04-25 Published:2010-04-26
  • Supported by:

    Foundation from Shanghai Jiaotong University School of Medicine, 06XJ21118；Shanghai Municipal Health Bureau Foundation, 2007164；Natural Science Foundation of Shanghai, 08ZR1421200

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摘要：

目的 分析炎症积水的输卵管上皮组织中Toll样受体（TLR）基因和关键信号转导分子的表达格局。方法 根据沙眼衣原体IgG检测(CAT)结果，将输卵管炎症积水患者分为CAT阳性组（n=20）和CAT阴性组（n=8）；另设正常对照组（n=13）。运用实时定量PCR方法，分析输卵管上皮组织中TLR1～10及NF-κB、MyD88、TRAF、IRAK mRNA的表达。结果 CAT阳性组和阴性组TLR2 mRNA表达均显著低于对照组(P<0.001)；而CAT阳性组与阴性组比较，差异无统计学意义(P>0.05)。CAT阳性组TLR4 mRNA表达显著低于对照组(P<0.001)和CAT阴性组(P<0.05)；而CAT阴性组与对照组比较，差异无统计学意义(P>0.05)。对照组MyD88 mRNA表达明显高于CAT阳性组(P<0.01)和CAT阴性组(P<0.05)；而CAT阳性组与阴性组比较，差异无统计学意义(P>0.05)。结论 输卵管炎症积水可能与既往输卵管感染后机体自身免疫损伤及感染局部TLR2、4介导的免疫反应不足有关。

关键词: 输卵管积水, 沙眼衣原体, IgG, Toll样受体, 信号转导

Abstract:

Objective To analyse the expression pattern of Toll-like receptor (TLR) and main signal transduction factors in tubal epithelial tissues with inflammatory hydrosalpinx. Methods According the results of serum Chlamydia trachomatisIgG antibody testing (CAT), patients with inflammatory hydrosalpinx were divided into CAT positive group (n=20) and CAT negative group (n=8), and normal control group (n=13) was also established. Real-time PCR was employed to detect the expression of TLR1-10 as well as NF-κB, MyD88, TRAF and IRAK mRNA in the fallopian tube epithelial tissues. Results The expression of TLR2 mRNA in CAT positive group and CAT negative group was significantly lower than that in control group (P<0.001), while there was no significant difference between CAT positive group and CAT negative group (P>0.05). The expression of TLR4 mRNA in CAT positive group was significantly lower than that in control group (P<0.001) and CAT negative group (P<0.05), while there was no difference between CAT negative group and control group (P>0.05). The expression of MyD88 in control group was significantly higher than that in CAT positive group (P<0.01) and CAT negative group (P<0.05), while there was no significant difference between CAT positive group and CAT negative group (P>0.05). Conclusion Autoimmunological injury after infection and the reduction of TLR2 and TLR4 in tubal tissues that likely induce an immunological deficiency are associated with hydrosalpinx following a tubal infection.

Key words: hydrosalpinx, Chlamydia trachomatis, IgG, Toll-like receptor, signal transduction