上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (10): 1204-.doi: 10.3969/j.issn.1674-8115.2010.10.005

• 论著（基础研究） • 上一篇    下一篇

低氘白酒对糖尿病大鼠糖代谢和胰岛细胞及其功能的影响

周振宇1, 沈才洪2, 卢中明2, 敖宗华2, 宋立华1, 丛峰松3   

  1. 1.上海交通大学 农业与生物学院, 上海 200240；2.泸州老窖集团有限责任公司, 泸州 646000； 3.上海交通大学 生命科学与技术学院, 上海 200240
  • 出版日期:2010-10-25 发布日期:2010-10-27
  • 通讯作者: 丛峰松, 电子信箱: fscong@sjtu.edu.cn；宋立华, 电子信箱: lihuas@sjtu.edu.cn。
  • 作者简介:周振宇(1985—), 男, 硕士生；电子信箱: johnnyzhou1985@126.com。

Effects of deuterium-depleted Chinese liquor on glucose metabolism and islet cells and their function of diabetic rats

ZHOU Zhen-yu1, SHEN Cai-hong2, LU Zhong-ming2, AO Zong-hua2, SONG Li-hua1, CONG Feng-song3   

  1. 1.School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China； 2.Luzhou Laojiao Limited Company, Luzhou 646000, China； 3.School of Life Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China
  • Online:2010-10-25 Published:2010-10-27

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摘要：

目的 探讨低氘白酒干预对糖尿病大鼠空腹血糖水平和胰岛细胞及其功能的影响。方法 一次性腹腔注射链脲佐菌素（STZ）诱导建立糖尿病Wistar大鼠模型，根据干预方式的不同将糖尿病大鼠分为模型组（生理盐水灌胃，饮用普通水）、低氘水组（低氘水灌胃，饮用低氘水）、普通白酒组\[1个单位（低量）或6个单位（高量）白酒+蒸馏水灌胃，饮用普通水）、低氘白酒组(低量或高量白酒+低氘水灌胃，饮用低氘水），每组10只；以10只未造模大鼠作为正常对照组（生理盐水灌胃，饮用普通水）；各组大鼠每日灌胃液体量均为0.01 mL/g。于干预后4周末，各组大鼠经眼眶静脉采集血样后颈椎脱臼处死，摘除并制备胰腺组织标本。葡萄糖氧化酶法和放射免疫分析法分别测定空腹血糖和胰岛素水平；胰腺组织标本HE染色观察各组大鼠胰岛组织病理学改变。结果 空腹血糖水平，普通白酒高量组>模型组＞普通白酒低量组>低氘白酒高量组>低氘水组>低氘白酒低量组，其中低氘水组和低氘白酒低量组与其他各组比较差异均有统计学意义(P<0.05)。空腹胰岛素水平，低氘白酒低量组>低氘白酒高量组>普通白酒低量组>低氘水组>普通白酒高量组>模型组；除普通白酒高量组外，其余各组均显著高于模型组（P<0.05）。胰岛组织学观察显示，与正常对照组比较，模型组和普通白酒组大鼠的胰岛细胞数量减少、体积增大、胞质呈空泡状，细胞排列紊乱，低氘水组和低氘白酒组胰岛细胞受损程度较轻。结论 低氘白酒干预能显著降低糖尿病大鼠的空腹血糖水平，提高血浆胰岛素水平；减轻因过量饮酒导致的胰岛细胞损伤。

关键词: 低氘水, 白酒, 糖尿病, 空腹血糖, 空腹胰岛素水平, 胰岛细胞

Abstract:

Objective To investigate the effects of deuterium-depleted Chinese liquor on fasting blood glucose (FBG) and islet cells and their function of diabetic rats. Methods Wister rat diabetic models were established by streptozotocin (STZ) one-time intraperitoneal injection. The experimental rats were divided into following groups (n=10) according to the means of intervention: model group (intragastric administration of normal saline, fed with normal water), deuteriumdepleted water (DDW) group (intragastric administration of DDW, fed with DDW), normal Chinese liquor group\[intragastric administration of one unit (low) or six unit (high) Chinese liquor and distilled water, fed with normal water\], deuterium-depleted Chinese liquor group (intragastric administration of low or high dose of Chinese liquor and DDW, fed with DDW), and control group (without STZ injection, intragastric administration of normal water, fed with normal water). An oral daily administration of liquor solution was 0.01 mL/g (volume/body weight). At the end of 4 weeks of intervention, the blood was collected from the orbital vein followed by killing the rats with the method of cervical dislocation. Then, pancreatic tissues were removed to make samples. The FBG was measured by glucose oxidase, and the fasting insulin levels (FINS) were analyzed by radioimmunoassay. The pathology structure of pancreatic islets was observed by HE staining. Results FBG in every group followed the order: normal Chinese liquor (high) group>model group>normal Chinese (low) liquor group>deuterium-depleted Chinese liquor (high) group>DDW group>deuterium-depleted Chinese liquor (low) group. And FBG of DDW group and deuteriumdepleted Chinese liquor (low) group had significant difference to other groups (P<0.05). FINS of every group followed the order: deuterium-depleted Chinese liquor (low) group>deuterium-depleted Chinese liquor (high) group>normal Chinese liquor (low) group>DDW group>normal Chinese liquor (high) group>model group. And FINS of other groups had significant differences to model group （P<0.05）except for the normal Chinese liquor (high) group. The observation of histological examination revealed that compared to control group, in model group and normal Chinese liquor group, the cell amount of pancreatic islets decreased and coupled with volume increase, vacuolar cytoplast, and disorder. While in DDW and deuterium-depleted Chinese liquor groups, pancreatic islet cells had mild damage. Conclusion The deuterium-depleted Chinese liquor has significant effects in decreasing FBG of rats, increasing FINS, and reduces the islet cells damage caused by excessive drinking.

Key words: deuterium-depleted water, Chinese liquor, diabetes mellitus, fasting blood glucose, fasting insulin levels, islet cell