上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (10): 1231-.doi: 10.3969/j.issn.1674-8115.2010.10.011

• 论著（临床研究） • 上一篇    下一篇

口腔白斑患者黏膜上皮组织中骨桥蛋白的表达

孙 巍1, 魏本娟1, 陆 群1, 周曾同2   

  1. 上海交通大学 医学院 1.附属仁济医院口腔科, 上海 200001； 2.附属第九人民医院口腔黏膜科, 上海 200011
  • 出版日期:2010-10-25 发布日期:2010-10-27
  • 通讯作者: 陆 群, 电子信箱： dentistry@126.com；周曾同, 电子信箱：zhouzengtong@hotmail.com。
  • 作者简介:孙 巍（1976—）, 男, 硕士生；电子信箱: dentist1609@hotmail.com；魏本娟（1973—）, 女, 主治医师, 博士；电子信箱：benjuanwei@yahoo.com.cn。
  • 基金资助:

    上海高校选拔培养优秀青年教师基金（JDY08036）

Expression of osteopontin in oral mucosal epithelium in patients with oral leukoplakia

SUN Wei1, WEI Ben-juan1, LU Qun1, ZHOU Zeng-tong2   

  1. 1.Department o Stomatology, Renji Hospital|Shanghai Jiaotong University School of Medicine, Shanghai 200001, China；2.Department of Oral Mucosa, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2010-10-25 Published:2010-10-27
  • Supported by:

    Foundation for the Excellent Youth Scholars of Higher Education of Shanghai, China, JDY08036

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摘要：

目的 研究口腔白斑（OLK）患者病损局部黏膜上皮细胞骨桥蛋白（OPN）表达情况。方法 选择经病理学检查确诊的20例OLK患者作为研究对象（OLK组），其中伴轻、中、重度上皮异常增生分别为9、8、3例。采集OLK病损局部黏膜组织标本，采用免疫组织化学方法检测黏膜上皮细胞OPN表达。以因外伤或美容需要而接受口内黏膜手术的20例非OLK患者作为对照组。结果 免疫组织化学染色观察发现，对照组口腔黏膜组织未见或仅见极少量上皮细胞胞质内呈现OPN阳性染色；OLK组伴重度上皮异常增生组织中OPN阳性细胞数量明显增多且分布于上皮全层。半定量分析显示，在伴有轻、中、重度上皮异常增生的OLK患者，病损局部黏膜上皮细胞OPN表达阳性率分别为77.8%、100.0%、100.0%，三者间比较差异无统计学意义（P>0.05)，但均显著高于对照组的10.0%（P<0.05）。结论 与正常口腔黏膜组织比较，OLK患者病损局部OPN 表达阳性率较高。提示OPN可能参与OLK的发生和癌变过程。

关键词: 口腔白斑, 骨桥蛋白, 免疫组织化学

Abstract:

Objective To explore the expression of osteopontin (OPN) in oral mucosal epithelium of oral local lesion in patients with oral leukoplakia (OLK). Methods Twenty patients with pathologically-confirmed OLK were selected (OLK group), including 9, 8, and 3 patients of mild, modern, and sever epithelium dysplasia, respectively. Mucosal epithelium tissues of OLK local lesion were collected and examined for the expression of OPN by immunohistochemical method. Twenty non-OLK patients were served as normal controls, who received oral mucosal operation because of accident injury or cosmetic surgery. Results In oral mucosal tissues of control group, there was no or very little OPN positive expression observed in epithelial cytoplasm by immunohistochemical staining. In OLK patients with sever epithelium dysplasia, the numbers of OPN positive cells increased obviously and the positive cells spread the whole epithelial cell stratum. The positive expression rates of OPN were 77.8%, 100% and 100% in OLK patients with mild, modern, and severe epithelium dysplasia by semi-quantitative analysis, respectively. There was no significant difference of positive expression rates among the three different dysplasia (P>0.05). But all of them were significantly higher than that in control group (11.1%) (P<0.05). Conclusion The expression of OPN significantly increased in mucosal epithelium of oral local lesion in patients with OLK, which indicates that OPN may be associated with the pathogenesis and carcinogenesis of OLK.

Key words: oral leukoplakia, osteopontin, immunohistochemistry