上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (11): 1616-.doi: 10.3969/j.issn.1674-8115.2011.11.024

• 论著（临床研究） • 上一篇    下一篇

正常孕妇和子前期患者胎盘缺氧诱导因子脯氨酸羟化酶的表达

刘 伟1, 林其德1, 王树军2   

  1. 上海交通大学 医学院1.附属仁济医院妇产科, 上海 200127； 2.免疫学研究所, 上海 200025
  • 出版日期:2011-11-28 发布日期:2011-11-29
  • 通讯作者: 林其德, 电子信箱: shlinqide@hotmail.com。
  • 作者简介:刘 伟（1969—）, 男, 副主任医师, 博士；电子信箱: liuwei947@hotmail.com。

Expression of hypoxia-inducible factor prolyl 4-hydroxylase in placentas of normal pregnant women and patients with pre-eclampsia

LIU Wei1, LIN Qi-de1, WANG Shu-jun2   

  1. 1.Department of Obstetrics and Gynaecology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China|2.Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2011-11-28 Published:2011-11-29

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摘要：

目的 分析正常孕妇和子前期患者胎盘组织缺氧诱导因子脯氨酸羟化酶（HPH）mRNA的表达，探讨子前期胎盘缺氧的发生与滋养叶细胞氧敏感性的关系。方法 行剖宫产分娩或接受计划生育手术的孕妇共66名，分为早孕组（n=13）、中孕组（n=9）、晚孕组（n=13，作为子前期组和妊娠期高血压组的对照）、子前期组（n=20）和妊娠期高血压组（n=11）。采用原位杂交和Real-Time PCR技术对胎盘或绒毛组织中HPH-1、-2、-3 mRNA的表达进行定位和定量检测。结果 HPH-1、-2、-3 mRNA主要在胎盘滋养叶细胞的细胞质内表达，HPH-1 mRNA在绒毛外滋养叶细胞的细胞质内呈强特异性表达。随着妊娠进展，HPH-1 mRNA表达量明显增加（r＝0.616，P<0.001）。子前期组HPH-1 mRNA表达量显著低于晚孕组（P<0.05）；妊娠期高血压组HPH-1 mRNA表达量也低于晚孕组，但差异无统计学意义（P>0.05）。子前期组患者胎盘质量与HPH-1 mRNA表达量呈正相关（r=0.457，P<0.05）。结论 子前期患者滋养叶细胞氧敏感性降低（HPH1 mRNA低表达）是导致胎盘缺氧表现（缺氧反应途径过度激活）的重要原因。

关键词: 子前期, 缺氧诱导因子脯氨酸羟化酶, 滋养叶细胞

Abstract:

Objective To investigate the expression of hypoxia-inducible factor prolyl 4-hydroxylase (HPH) in placentas of normal pregnant women and patients with pre-eclampsia, and explore the relationship between oxygen sensitivity of trophoblast and hypoxia in preeclamptic placenta. Methods Sixty-six pregnant women undergoing cesarean section or family planning surgery were divided into early pregnancy group (n=13), mid-pregnancy group (n=9), late pregnancy group (n=13, controls for pre-eclampsia group and gestational hypertension group), pre-eclampsia group (n=20) and gestational hypertension group (n=11). The expression of HPH-1, HPH-2 and HPH-3 mRNA in placentas and villous tissues was determined by in situ hybridization and Real-Time PCR. Results HPH-1, HPH-2 and HPH-3 mRNA was mainly expressed in cytoplasm of trophoblast, and HPH-1 mRNA was significantly expressed in cytoplasm of extravillous trophoblast. With the progress of pregnancy, the expression of HPH-1 mRNA significantly increased (r=0.616, P<0.001). The expression of HPH-1 mRNA in pre-eclampsia group was significantly lower than that in late pregnancy group (P<0.05). The expression of HPH-1 mRNA in gestational hypertension group was also lower than that in late pregnancy group, while there was no significant difference between them (P>0.05). The weight of placentas in pre-eclampsia group was significantly related to the expression of HPH-1 mRNA (r=0.457, P<0.05). Conclusion Low oxygen sensitivity of trophoblast (low expression of HPH-1 mRNA) may be an important cause for placenta hypoxia (overactivation of hypoxia reaction pathway) in patients with pre-eclampsia.

Key words: pre-eclampsia, hypoxia-inducible factor prolyl 4-hydroxylase, trophoblast