上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (12): 1719-.doi: 10.3969/j.issn.1674-8115.2011.12.013

• 论著（基础研究） • 上一篇    下一篇

同种异体肺和复合组织序贯移植大鼠模型研究

王守宝1, 张睿智2, 刘 菲1, 罗旭松1, 杨 军1, L. Scott Levin3   

  1. 1.上海交通大学 医学院附属第九人民医院整形外科, 上海 200011； 2.台湾佛教慈济综合医院胸心血管外科, 花莲 94848325； 3.美国宾夕法尼亚大学附属医院整形外科, 费城 215-829-3461
  • 出版日期:2011-12-28 发布日期:2012-01-04
  • 通讯作者: 罗旭松, 电子信箱: luoxs71@126.com。
  • 作者简介:王守宝（1986—）, 男, 硕士生；电子信箱: wangshoubao@msn.com；张睿智（1977—）, 男, 博士生；电子信箱: medraytw@hotmail.com。

Successive composite tissue allotransplantation on lung allotransplanted rat model

WANG Shou-bao1, Jui-Chih Chang2, LIU Fei1, LUO Xu-song1, YANG Jun1, L. Scott Levin3   

  1. 1.Department of Plastic and Reconstructive Surgery, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China； 2.Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Buddhist Tzu Chi General Hospital, Hualien 94848325, China； 3.Department of Orthopaedic Surgery, the Hospital of the University of Pennsylvania, Philadelphia 215-829-3461, USA
  • Online:2011-12-28 Published:2012-01-04

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摘要：

目的 探讨大鼠同种异体序贯复合组织移植（SCTA）对先前实质器官移植免疫效应的影响。方法 取WKY大鼠左肺原位移植到F344大鼠，应用Cuff技术分别吻合左肺动脉、左肺静脉及主支气管。术后对F344大鼠应用环孢素（CsA）5 mg/kg隔日皮下注射，共10 d；以呼吸频率检测移植左肺功能。肺移植后第11天，取Brown Norway大鼠腹部3 cm×3 cm肌皮瓣原位移植至F344大鼠，皮瓣吻合血管为股动脉和股静脉，皮瓣移植后停止应用免疫抑制剂。同种异体复合组织移植（CTA）10 d后，取移植皮瓣和肺组织行组织病理学检查，观察排斥反应。结果 大鼠肺移植手术成功率90%，CTA手术成功率100%，SCTA成功率90%。移植肺的呼吸频率从移植10 d后（应用免疫抑制剂10 d）的（61±10）次/min增加到移植20 d后（停用免疫抑制剂10 d）的（75±15）次/min。移植肺显示出典型的急性免疫排斥反应，包括血管周围及肺组织内弥漫的淋巴细胞浸润。自体移植腹壁皮瓣病理组织学检查未发现免疫排斥反应，CTA术后皮瓣显示典型血管周围及皮瓣内弥漫的淋巴细胞浸润。结论 SCTA大鼠模型的建立对于实质器官移植后CTA及二次移植免疫抑制方案的研究具有重要意义。

关键词: 序贯移植, 同种异体复合组织移植, 肺移植, 大鼠模型

Abstract:

Objective To investigate the immunosuppression effect of successive composite tissue allotransplantation（SCTA）after previous parenchymatous organ transplantation in rats. Methods Left lungs from WKY rats were orthotopically transplanted into F344 rats, and cuff technique was employed for anastomoses of the left pulmonary vein, left pulmonary artery and main bronchus. F344 rats were subcutaneously injected with cyclosporine A (5 mg/kg) once every two days for 10 d after operation. Transplanted pulmonary function was assessed with respiratory rate. On the eleventh day after lung transplantation, 3 cm×3 cm abdominal musculocutaneous flaps harvested from Brown Norway rats were transplanted to F344 rats, and the abdominal flap vessels were connected to the femoral vessels. No further immunosuppression was administered following flap transplantion. Ten days after composite tissue allotransplantation (CTA), transplanted flaps and lung tissues were obtained for histopathological examinations, and rejection reaction was observed. Results The successful rates of lung transplantation, CTA and SCTA were 90%, 100% and 90% respectively. The respiratory rates increased from (61±10) times/min on day 10 after transplantation (immunosuppression for 10 d) to (75±15) times/min on day 20 after transplantation (immunosuppression withdrawl for 10 d). The allotransplanted lung exhibited typical signs of acute rejection, including both perivascular and diffuse lymphocytic infiltration. There was no acute rejection in abdominal flap autograft by histopathological observation, and the allotransplanted abdominal flaps had typical perivascular and diffuse lymphocytic infiltration after CTA. Conclusion SCTA rat model may facilitate the research of secondary CTA following primary parenchymatous organ transplantation and can be useful in developing future immunotherapeutic strategies.

Key words: successive transplantation, composite tissue allotransplantation, lung transplantation, rat model