上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (10): 1312-.doi: 10.3969/j.issn.1674-8115.2012.10.007

• 论著（基础研究） • 上一篇    下一篇

18F-FLT与18F-FDG评估白血病荷瘤裸鼠模型肿瘤增殖的研究

路丽彦, 姜 磊, 高云朝, 陆汉魁   

  1. 上海交通大学附属第六人民医院核医学科, 上海 200233
  • 出版日期:2012-10-28 发布日期:2012-11-05
  • 通讯作者: 陆汉魁, 电子信箱: luhk163@163.com。
  • 作者简介:路丽彦（1986—）, 女, 硕士生；电子信箱: luliyan.ok@163.com。

Application of 18F-FLT and 18F-FDG in evaluation of tumor proliferation of nude mouse models of leukemia

LU Li-yan, JIANG Lei, GAO Yun-chao, LU Han-kui   

  1. Department of Nuclear Medicine, the Sixth People´s Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2012-10-28 Published:2012-11-05

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摘要：

目的 对比研究3´-脱氧-3´-18F-氟代胸苷（18F-FLT）和18F-氟代脱氧葡萄糖（18F-FDG）在评估慢性髓性白血病（K562细胞株）荷瘤裸鼠模型肿瘤增殖中的应用价值。方法 合成放射性显像剂18F-FLT，常规体外培养K562细胞株，分别于加入18F-FLT和18F-FDG后15、30、60 和120 min，测定细胞对18F-FLT与18F-FDG的摄取率。将K562细胞株接种于裸鼠肩部皮下建立白血病荷瘤裸鼠模型，并经鼠尾静脉注射18F-FLT和18F-FDG，分别于注射后30、60 和120 min，行正电子发射型计算机断层显像（PET），计算靶/非靶（T/NT）比值。结果 所合成的18F-FLT经高效液相色谱纯化，放置6 h内放射化学纯度＞95%。病理学检查显示经皮下接种K562细胞株可以成功建立白血病荷瘤裸鼠模型；体外细胞摄取试验结果显示：不同时间点K562细胞对18F-FLT的摄取率均显著高于对18F-FDG的摄取率(P＜0.05)；PET显像示：不同显像时间K562移植瘤18F-FLT PET显像组T/NT比值分别高于18F-FDG PET显像组T/NT比值(P＜0.05)。结论 与显像剂18F-FDG相比较，18F-FLT能更好地反映白血病荷瘤裸鼠模型移植肿瘤的增殖情况。

关键词: 胸苷, 脱氧葡萄糖, 体层摄影术, 发射型计算机, 白血病, 小鼠, 裸

Abstract:

Objective To investigate the application of 18F-fluoro-3´-Deoxy-3´-L-fluorothymidine (18F-FLT) and 18F-fluorodeoxyglucose (18F-FDG) in evaluation of tumor proliferation of nude mouse models of chronic myelogenous leukemia(K562 cell lines). Methods 18F-FLT was synthesized and K562 cells were traditionally cultured. The in vitro experiment of 18F-FLT and 18F-FDG uptake assay was performed in K562 cells at 15 min, 30 min, 60 min and 120 min. After tumorbearing mouse models of leukemia were successfully established by injection of K562 cells, positron emission computed tomography (PET) was performed 30 min, 60 min and 120 min after injection of 18F-FLT and 18F-FDG. The region of interest was drawn over the tumor and background to calculate the ratio of tumor to non-tumor (T/NT). Results High-performance liquid chromatography indicated that the radiochemical purity of 18F-FLT was over 95% within 6 h. Pathological examination revealed that tumor-bearing nude mouse models of leukemia were successfully established by subcutaneous inoculation of K562 cells. A significantly higher uptake of 18F-FLT than that of 18F-FDG was observed in K562 cells in the tracer uptake experiment at various time points (P＜0.05). And ratios of T/NT of 18F-FLT PET were significantly higher than those of 18F-FDG PET at different time points after injection (P＜0.05). Conclusion Compared with 18F-FDG, 18F-FLT may have better performance in evaluation of tumor proliferation of nude mouse models of leukemia.

Key words: thymidine, deoxyglucose, tomography, emission-computed, leukemia, mice, nude