›› 2013, Vol. 33 ›› Issue (2): 145-.doi: 10.3969/j.issn.1674-8115.2013.02.004

• 论著(基础研究) • 上一篇    下一篇


侯雪峰1, 宋小飞2, 王朝霞1, 戴靖波1, 唐茂萍1, 陈晓辉1, 宋肖静3, 高 萍1, 乔中东1   

  1. 1.上海交通大学 生命科学技术学院, 上海 200240; 2.上海瑞金集团闵行中心医院泌尿外科, 上海 201100; 3.山西医科大学细胞生物学教研室, 太原 030001
  • 出版日期:2013-02-28 发布日期:2013-03-07
  • 通讯作者: 王朝霞, 电子信箱: zhaoxiaw@sjtu.edu.cn。
  • 作者简介:侯雪峰(1989—), 女, 硕士生; 电子信箱: xueyuefenghua@sjtu.edu.cn; 宋小飞(1982—), 男, 硕士; 电子信箱: sxfonline@qq.com。
  • 基金资助:


Anti-skin aging effect of GX-50

HOU Xue-feng1, SONG Xiao-fei2, WANG Zhao-xia1, DAI Jing-bo1, TANG Mao-ping1, CHEN Xiao-hui1, SONG Xiao-jing3, GAO Ping1, QIAO Zhong-dong1   

  1. 1.School of Life Sciences and Technology, Shanghai Jiaotong University, Shanghai 200240, China; 2.Department of Urology, Minhang Central Hospital of Ruijin Group, Shanghai 201100, China;3.Department of Cytology, Shanxi Medical University, Taiyuan 030001, China
  • Online:2013-02-28 Published:2013-03-07
  • Supported by:

    530 Project of Wuxi, Jiangsu Province, 2011JDZX020


目的 研究GX-50抗皮肤衰老的作用。方法 通过在20月高龄小鼠背部涂抹GX-50,采用HE染色和免疫组织化学法对GX-50抗皮肤衰老的作用进行组织学观察。原代培养人皮肤成纤维细胞,采用H2O2诱导建立皮肤细胞衰老模型,分为空白对照组、DMSO组、GX-50组、H2O2组和GX-50预处理后H2O2处理组(GX-50+H2O2组);采用β-半乳糖苷酶染色实验以及测定细胞分泌物丙二醛(MDA)和细胞内活性氧(ROS)含量检测GX-50在细胞水平的抗衰老能力。应用Draize兔眼刺激性实验评价GX-50的刺激性。结果 在组织学水平,GX-50可使高龄小鼠衰老的皮肤年轻化,增加胶原蛋白的表达。在细胞水平,GX-50可使人皮肤成纤维细胞β-半乳糖苷酶合成降低;GX-50组细胞MDA分泌量较空白对照组下降30.45%,GX-50+H2O2组较H2O2组下降61.46%,组间比较差异均有统计学意义(P<0.05);GX-50组细胞胞内ROS荧光值较空白对照组下降8.03%,GX-50+H2O2组较H2O2组下降8.36%,组间比较差异均有统计学意义(P<0.05)。Draize兔眼刺激性评分为0。结论 GX-50可以在一定程度上保护和延缓皮肤衰老,具有作为护肤品有效添加剂的应用前景。

关键词: GX-50, 皮肤衰老, 胶原蛋白, 丙二醛, 活性氧


Objective To investigate the protective effect of GX-50 against skin aging. Methods GX-50 was applied on the back of mice aged 20 months, and the effect of GX-50 on mouse skin was observed with HE staining and immunohistochemistry. Human skin fibroblasts were primarily cultured, senescent cell model was established by H2O2 induction, and blank control group, DMSO group, GX-50 group, H2O2 group, and GX-50+H2O2 group (pretreatment with GX-50+H2O2 treatment) were divided. The anti-aging effect of GX-50 on primary human skin fibroblasts was examined by SA-βGal staining, as well as determination of secreted malondialdehyde (MDA) concentration and intracellular reactive oxygen species (ROS) content. The irritation characteristics of GX-50 were detected by Draize eye test. Results The histological observation revealed that the GX-50 treated skin exhibited better appearance and higher collagen content. β-galactosidase in human skin fibroblasts was reduced by GX-50. Compared with blank control group, the secretion of MDA was down-regulated by 30.45% and the fluorescence value of ROS was down-regulated by 8.03% in GX-50 group (P<0.05). Compared with H2O2 group, the secretion of MDA was down-regulated by 61.46% and the fluorescence value of ROS was down-regulated by 8.36% in GX-50+H2O2 group (P<0.05). Draize eye test showed the irritation score of GX-50 was 0. Conclusion GX-50 has protective and anti-aging effect on skin, and may serve as a potential cosmetic additive against skin aging.

Key words: GX-50, skin aging, collagen, malondialdehyde, reactive oxygen species