›› 2018, Vol. 38 ›› Issue (3): 259-.doi: 10.3969/j.issn.1674-8115.2018.03.004

• Original article (Basic research) • Previous Articles     Next Articles

Antibacterial effect of Nd: YAG laser on Fusobacterium nucleatum

WU Yi-fan, SONG Zhong-chen, SHU Rong   

  1. Shanghai Key Laboratory of Stomatology, Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2018-03-28 Published:2018-05-03
  • Supported by:
    National Nature Science Foundation of China, 81570977

Abstract: Objective · To investigate the antibacterial effect of Nd: YAG laser on Fusobacterium nucleatum (F. nucleatum) in vitro. Methods · The
laser effect on the biofilm formation ability of planktonic F. nucleatum were observed by crystal violet test; confocal laser scanning microscopy (CLSM)
was used to detect the effect on the bacteria viability of mature biofilm; scanning electron microscopy (SEM) was performed to investigate the effect on
the morphology of mature bioflm. Results · After 15 s radiation the biofilm formation ability of F. nucleatum went down. The viability of F. nucleatum
in mature biofilm went down under 35 s iradiation. The deformation of mature biofilm and bacterium became distincted with the iradiation time raised.
Conclusion · Nd: YAG laser shows the inhibition of biofilm formation ability on planktonic F. nucleatum. The vitality of F. nucleatum in mature biofilm is
depressed after laser radiation. Laser can destroy the construction of the mature biofilm and bacteria.

Key words: periodontal pathogens, biofilm, Nd: YAG laser, in vitro experiment