›› 2019, Vol. 39 ›› Issue (2): 113-.doi: 10.3969/j.issn.1674-8115.2019.02.002

• Original article (Basic research) • Previous Articles     Next Articles

Differential analysis of schizophrenia susceptibility genes in single cells of human and mobrain

CHENG Ying1, JU Pei-jun1, WANG Wei-di1, FANG Yu1, LIN Guan-ning1, 2, CUI Dong-hong1   

  1. 1. Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China; 2. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China
  • Online:2019-02-28 Published:2019-03-19
  • Supported by:
    National Key Research and Development Plan, 2017YFC0909200; National Natural Science Foundation of China, 81671336; Shanghai Committee of Science and Technology Research Project, 13dz2260500

Abstract: Objective · To investigate the patterns of schizophrenia susceptibility genes in different neuronal cell types of human and mobrains. Methods · Schizophrenia susceptibility genes were studied based on four genetic study methods, including genome-wide association study, linkage and association study, copy number variation study and convergent functional genomics study. Single cell RNA-seq data of human and mobrains were used to explore the patterns of schizophrenia susceptibility genes in specific cell types of neurons, astrocytes, microglia, oligodendrocytes, oligodendrocyte progenitor cells. Furthermore, the functions of schizophrenia risk genes identified only in human brains were analyzedfunctional annotation tools the DAVID database. Results · Comparisons were made about single cell RNA-seq data between human and mobrains, and there existed distinct patterns of schizophrenia susceptibility genes across species. Neurons, astrocytes and oligodendrocytes both human and mowere shown to have more co-expressed schizophrenia susceptibility genes, while co- of schizophrenia susceptibility genes in microglia and oligodendrocyte progenitor cells rarely existed. In addition, schizophrenia risk genes expressed only in human were involved in the regulation of neural synaptic plasticity and calcium signaling pathway. Conclusion · The schizophrenia susceptibility genes have distinct profiles at the single cell level in human and mobrains, which provides clues and evidence for revealing the etiological mechanism of schizophrenia based on momodel research.

Key words: schizophrenia, susceptibility gene, momodel, single cell RNA-seq, gene

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