›› 2020, Vol. 40 ›› Issue (4): 437-.doi: 10.3969/j.issn.1674-8115.2020.04.004

• Original article (Basic research) • Previous Articles     Next Articles

Effect of indoxyl sulfate on proliferation and of inflammatory cytokines and ROS in human periodontal ligament cells

XIA Xin-yi, CHEN Hui-wen, HU Yi, SONG Zhong-chen   

  1. Department of Periodontology, Shanghai Ninth Peoples Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China
  • Online:2020-04-28 Published:2020-05-22
  • Supported by:
    National Key R&D Program of China (2017YFC0840100, 2017YFC0840110).

Abstract: Objective · To explore the effects of Indoxyl sulfate (IS) on proliferation activity, of inflammatory factors and reactive oxygen species (ROS) in human periodontal ligament cells (hPDLCs). Methods · The primary hPDLCs were culturedusing tissue explant method in vitro. MTT assay was employed to evaluate the effect of IS on proliferation activity of hPDLCs. The mRNA and protein s of IL-1β, IL-6, and IL-8 were detectedusing real-time PCR and ELISA assay. DCFDA fluorescence probe was used to detect intracellular ROS and ROS in the cytoplasm under fluorescence microscope. Results · The viability of hPDLCs was inhibitedIS at the concentration of 125 μmol/L on 24, 48 and 72 hours. The inhibitory effect was presented in a dose- and time- dependent manner. IS could upregulate the mRNA and protein of inflammatory cytokines including IL-1β, IL-6 and IL-8 as well as promote ROS in hPDLCs. Conclusion · IS may play an important role in the association between chronic kidney diseases and periodontitis through inhibiting the activity of hPDLCs, promoting the of inflammatory cytokines and increasing intracellular ROS level.

Key words: periodontitis, chronic renal failure, indoxyl sulfate