JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2020, Vol. 40 ›› Issue (10): 1360-1364.doi: 10.3969/j.issn.1674-8115.2020.10.010

• Original article (Basic research) • Previous Articles     Next Articles

Effects of periodontitis on the subsets of helper T cells in peripheral blood of rat models

YUAN Yun, ZHANG Hong-ming, HUANG Hui   

  1. Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China
  • Online:2020-10-28 Published:2020-11-27
  • Supported by:
    National Natural Science Foundation of China (81771105).

Abstract: Objective · To investigate the effects of periodontitis on the subsets of helper T (Th) cells in peripheral blood of rat models, so as to provide basis for the immunological study of periodontitis. Methods · Rat models of experimental periodontitis were induced by gingiva separation and silk tread ligation. They were divided into periodontitis group (n=5) and normal group (control group, n=5). Periodontal probing was performed two weeks after operation. Alveolar bone resorption was measured by micro-computed tomography (micro-CT). Peripheral blood was taken for flow cytometry to detect the proportions of Th1, Th2, Th17 and T-regulatory (Treg) cells. Difference of cell proportion between the two groups was performed with independent sample t-test. Results · The gingival tissue was swollen and easy to bleed, and alveolar bone resorption was obvious in the periodontitis group. Flow cytometry showed that the proportions of Th1 (P=0.002) and Th17 (P=0.028) cells in peripheral blood of the periodontitis group significantly increased, the proportion of Treg cells significantly decreased (P=0.002), and the proportion of Th2 cells did not change obviously. Conclusion · Periodontitis promotes the differentiation of pro-inflammatory Th cell subsets, while inhibiting the differentiation of inflammation-regulating Th cell subset in peripheral blood of rat models.

Key words: periodontitis, helper T cell, rat, Th17 cell, T-regulatory cell

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