·Firstly, 177 patients who were hospitalized in the Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine from September 2019 to December 2019 and were diagnosed with coronary artery obstruction by coronary angiography were selected. According to the Rentrop method, the patients were classified into two groups: poor collateral circulation formation group and good collateral circulation formation group. ELISA was used to detect the level of adipokine complement CTRP2 in patients′ plasma. The animal model of lower limb ischemia was established and divided into contrast group and CTRP2 overexpression group. The recovery of blood supply was measured by laser Doppler instrument and the recovery ratio of blood supply was calculated. Finally, the recombinant CTRP2 protein was used to stimulate vascular endothelial cells at the doses of 50, 100 and 200 pg/mL, and the percentage was detected on Matrix Gel. The migration ability of endothelial cells was detected by Trans-well.

·Clinical study found that the plasma CTRP2 level in patients having coronary artery occlusion with good collateral formation was higher than that in patients with poor collateral circulation (P=0.009), and the risk ratio of CTRP2 to poor collateral circulation was OR=0.889, 95%CI 0.844?0.936, P=0.000. The animal model study found that CTRP2 can promote the recovery of blood supply in mice with lower limb ischemia (P=0.032, P=0.009). Cell experiments showed that CTRP2 enhanced the angiogenesis and migration ability of endothelial cells, and it was positively correlated with its concentration (all P<0.05).

·The level of plasma CTRP2 in patients having coronary artery occlusion with good collateral circulation is increased. CTRP2 can promote the establishment of collateral circulation in vivo and the angiogenesis and migration of endothelial cells in vitro.