›› 2009, Vol. 29 ›› Issue (10): 1174-.

• Original article (Basic research) • Previous Articles     Next Articles

Effects of photochemotherapy on angiogenic ability of endothelial cells and expression of integrin

DENG Hui1, |YUAN Ding-fen1, |YAN Chun-lin2, |LIAO Kang-huang2   

  1. 1. Department of Dermatology, The Sixth people's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；2. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Online:2009-10-25 Published:2009-10-26
  • Supported by:

    Shanghai Education Committee Foundation, 01QN03

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Abstract:

Objective To investigate the effects of photochemotherapy on the angiogenic ability of endothelial cells and expression of integrin. Methods In vitro angiogenesis assay (UVA exposure dose: 0, 2.0, 5.0 J/cm2) was used to detect the effects of photochemotherapy on the angiogenic ability of endothelial cells. Reverse polymerase chain reaction and flow cytometry were employed to determine the effects of photochemotherapy on the expression of integrin mRNA and protein, respectively. bFGF stimulation group and PMA stimulation group were divided according to the inductors, and subgroups were divided according to the UVA exposure doses of 0, 2.0 and 5.0 J/cm2 in each group. Results Combination of UVA (2.0 and 5.0 J/cm2) and 8-MOP (100 ng/mL) resulted in a decrease in the angiogenic ability of endothelial cells in vitro and the expression of integrin mRNA and protein. Conclusion Photochemotherapy may inhibit the angiogenic ability of endothelial cells through downregulating the expression of integrin.

Key words: psoriasis, angiogenesis, photochemotherapy, integrin

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