Abstract:

Objective To investigate the changes of protein expression during neuronal aging. Methods The experimental models of neuronal aging were established using serum-free culture of mouse neuroblastoma cells and were divided into 12 d group (aging group) and 5 d group (control group). The protein levels which changed during aging were analysed and identified using two-dimensional electrophoresis with matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Results Compared with 5 d group, the expression of 19 proteins increased, and that of 21 proteins decreased in 12 d group. Twelve proteins were successfully identified. The expression of VIME, DLDH, FSCN1 and GSTA4 increased, and that of TPM4, STMN1, TERA, PSA5, HSP90B, SERB, SYG and PARK7 decreased in 12 d group. Conclusion The molecular mechanisms of neuronal aging are involved in regulation of proteins such as STMN1, PARK7 and DLDH.

Key words: neuron, aging, proteomics