Abstract:

Objective To observe the therapeutic effects and explore the mechanism of anisodamine on coronary microcirculatory disturbance by establishing coronary microthrombosis models in New Zealand white rabbits. Methods A total of 24 New Zealand white rabbits were randomly divided into control group, model group and treatment group, with 8 rabbits in each group. In model group, coronary microthrombosis models were established by injection of sodium laurate into aortic root at the dose of 1.5 mg/kg (40 mg/mL) with aortic clamping for 10 s. In treatment group, 10 mg of anisodamine was administered by intramuscular injection after injection of sodium laurate. In control group, no treatment was performed. Then, 2 mL blood samples were obtained from ear artery before operation and 3 h after operation in model group and treatment group, while the blood samples in control group were obtained at randomized time points with the interval of 3 h. The serum concentrations of nitric oxide (NO), endothelin-1(ET-1), vascular endothelial growth factor (VEGF) and cardiac troponinⅠ (cTnⅠ) were measured. Animals were sacrificed, pathological sections of heart were prepared, the formation of microthrombus was observed, and the rates of microthrombus formation  were calculated. Results Three hours after injection of sodium laurate in model group, the serum concentration of NO was lower than that before operation, and the concentrations of ET-1, VEGF and cTn I were higher. However, the tendency of changes of NO, ET-1, VEGF and cTnⅠ in treatment group was in line with model group, and  were more moderate. There were significant differences between treatment group and model group in the changes of each parameter before operation and after operation (P<0.01). Three hours after injection of sodium laurate, NO/ET-1 ratio of treatment group was significantly higher than that of model group (P<0.05). There were no thromboses in coronary arteries in each group and no thromboses in arterioles in control group, while there were typical thromboses in arterioles in model group and treatment group. The rate of microthrombus formation in treatment group was lower than that in model group (P<0.05). Conclusion Anisodamine can effectively improve microcirculations, reduce the damage of endothelial cells, correct the imbalance of NO/ET-1, thereby reduce microthrombus induced by sodium laurate, and ultimately, effectively remedy coronary microcirculatory disturbance.

Key words: anisodamine, sodium laurate, coronary artery, microcirculation, microthrombus