›› 2011, Vol. 31 ›› Issue (10): 1492-.doi: 10.3969/j.issn.1674-8115.2011.10.030

• Brief original article • Previous Articles     Next Articles

Detection of amount of Aflatoxin B1 in rice samples in Shanghai

YE Lu, WEI Yan-xia, LIU Chang   

  1. Department of Medical Microbiology and Parasitology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2011-10-28 Published:2011-10-27

Abstract:

Objective To determine and analyse the amount of Aflatoxin B1 in rice samples sold in Shanghai. Methods Subei and Dongbei rice samples were obtained from groceries, medium-size markets, supermarkets and e-shops, and the amount of Aflatoxin B1 in rice samples was determined by enzyme linked immunosorbent assay. The amount of Aflatoxin B1 in Subei and Dongbei rice samples was compared by T test, and the amount of Aflatoxin B1 in rice samples obtained through different methods was compared by one-way analysis of variance. Results The mean amount of Aflatoxin B1 in all the 60 samples was 1.926 ppb. The amount of Aflatoxin B1 in 2 samples was higher than the national standard, which were Dongbei rice samples obtained from a grocery and a medium-size market. The amount of Aflatoxin B1 in Subei rice and Dongbei rice samples was (1.221±1.055) ppb and (2.631±2.903) ppb respectively, with significant differences between them (P<0.05). The amount of Aflatoxin B1 in rice samples obtained from groceries, e-shops, medium-size markets and supermarkets was (2.900±2.457) ppb, (1.570±1.416) ppb, (1.743±2.683) ppb and (0.569±0.608) ppb respectively, with significant differences among them (P<0.05). Multiple test revealed that there were significant differences in the amount of Aflatoxin B1 between rice samples obtained from groceries and those obtained from supermarkets (P<0.05). Conclusion The amount of Aflatoxin B1 in rice samples sold in Shanghai is relatively lower. The amount of Aflatoxin B1 in Dongbei rice samples is higher than that in Subei rice samples, and the amount of Aflatoxin B1 in rice samples obtained from groceries is higher than that obtained from supermarkets. Unqualified rice samples are obtained from a grocery and a medium-size market.

Key words: Aflatoxin B1, rice, enzyme linked immunosorbent assay