›› 2013, Vol. 33 ›› Issue (4): 421-.doi: 10.3969/j.issn.1674-8115.2013.04.008

• Original article (Basic research) • Previous Articles     Next Articles

Effects of blocking Notch3 pathway by siRNA under hypoxic condition on proliferation of human lung adenocarcinoma A549 cells

HAN Rui-chao1,2, FAN Zhi-wen3, ZHOU Min1,3   

  1. 1.Department of Respiratory Medicine, Jinshan Branch of the Sixth People´s Hospital, Shanghai Jiaotong University, Shanghai 201500, China； 2.Department of Respiratory Medicine, Xinhua hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China； 3.Department of Respiratory Medicine, the Third People´s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China
  • Online:2013-04-28 Published:2013-05-03
  • Supported by:

    Shanghai Jinshan District Science and Technology Innovation Foundation, 2011-3-06； Shanghai Education Committee Foundation,11YZ44

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Abstract:

Objective To observe the effects of blocking Notch3 by siRNA under hypoxic condition on the proliferation of human lung adenocarcinoma A549 cells. Methods Human lung adenocarcinoma A549 cells were cultured under hypoxic condition (1% O2). After transfection with Notch3 siRNA (Notch3 siRNA group), RT-PCR and Western blotting were used to detect the expression of Notch3 and its downstream gene HES1 mRNA and protein in A549 cells respectively, and the cell viability 24 h, 48 h and 72 h after transfection was determined by MTT assay. Besides, negative control group and blank control group were established. Results After transfection with Notch3 siRNA, the expression of Notch3 and HES1 mRNA and protein in A549 cells was significantly decreased （P<0.05）. Transfection with Notch3 siRNA time-dependently inhibited the growth of A549 cells, and the cell viability 48 h and 72 h after transfection in Notch3 siRNA group was significantly different from those in negative control group and blank control group （P<0.05）. Conclusion Transfection with Notch3 siRNA under hypoxic condition may effectively inhibit the expression of Notch3 and its downstream gene HES1 in human lung adenocarcinoma A549 cells, and may suppress the growth of A549 cells.

Key words: Notch3, lung adenocarcinoma, siRNA, hypoxia