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Inhibitory effect of 5-fluorouracil combined with Rg3 on proliferation of gastric cancer cells

NIE Na1, WU Xiao-ling2, LUO Hong-chun3, LIU Bo4, LIU Qian5   

  1. 1.Jinshan Hospital Medical Center， the First Affiliated Hospital of Chongqing Medical University, Chongqing 401122, China; 2.Department of Gastroenterology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; 3.Department of Infection, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 4.Department of Gastroenterology, the Children Affiliated Hospital of Chongqing Medical University, Chongqing 400014, China; 5.Department of Gastroenterology, Yubei People's Hospital of Chongqing, Chongqing 401120, China
  • Online:2014-05-28 Published:2014-05-30

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Abstract:

Objective To investigate the inhibitory effect of 5-fluorouracil (5-FU) combined with Rg3 on the proliferation of gastric cancer cells. Methods The inhibitory effect of 5-FU combined with Rg3 on the proliferation of human gastric cancer cells MGC-803 and MKN-28 was investigated by the MTT assay. The in vitro tumor spheroid model was built for studying the inhibitory effect of 5-FU combined with Rg3 on the growth of tumor spheroid. The gastric xenograft tumor model of nude mice was built and mice were randomly divided into the saline group (negative control group), 5-FU group, Rg3 group, and 5-FU+Rg3 group (combined administration group). The inhibitory effect of 5-FU combined with Rg3 on the growth of xenograft tumor of nude mice was investigated. Tumor sizes of each group were measured and growth curves of tumors were plotted. Results The viability of MGC-803 cells and MKN-28 cells of the combined administration group was significantly lower than that of the other groups after being administrated by 5-FU and Rg3 for 48 h. The difference was statistically significant (P<0.01). The sizes of tumor spheroid of MGC-803 cells and MKN-28 cells of the combined administration group were significantly smaller than those of the other groups after being administrated by 5-FU and Rg3 for 7 d. The difference was statistically significant (P<0.01). The results of apoptosis tests showed that the ability of inducing apoptosis of the combined administration group was significantly stronger than that of the other groups. The difference was statistically significant (P<0.01). The results of tumor bearing nude mice tests showed that each administration group could inhibit the growth of tumors. Compared to the negative control group, the differences were statistically significant (P<0.01). The combination of 5-FU and Rg3 could prolong the median survival time of tumor bearing nude mice. The difference was statistically significant (P<0.01). Conclusion Both 5-FU and Rg3 can inhibit the growth of gastric cancer cells and the combination of 5-FU and Rg3 may be a potentially effective treatment method for gastric cancer.

Key words: 5-fluorouracil, Rg3, gastric cancer