›› 2010, Vol. 30 ›› Issue (3): 288-.

• Original article (Basic research) • Previous Articles     Next Articles

Expression of CypB in colorectal cancer tissues and its effects on |metastasis and invasion of cancer cells

YUE Fei, ZHENG Min-hua, LU Ai-guo, LI Jian-wen, WANG Ming-liang   

  1. Department of General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-03-25 Published:2010-03-24
  • Supported by:

    National Natural Science Foundation of China, 30873000;Ph.D. Grant of Shanghai Jiaotong University School of Medicine, BXJ0807

Abstract:

Objective To investigate the expression of cyclophilin B (CypB) in colorectal cancer tissues with or without lymph node metastasis, and explore the effects of CypB plasmids knockdown on metastasis and invasion of cancer cells. Methods The expression of CypB in 59 colorectal cancer tissue microarray, including 31 without lymph node metastasis and 28 with lymph node metastasis, were evaluated with immunohistochemistry. Colorectal cancer cell line SW1116 was transfected with shRNA plasmids targeting CypB(SW1116-siCypB) and negative control plasmids(SW1116-NC). CypB knockdown effects were analysed with Western blotting. Wound healing assay and transwell cell invasion assay were performed to observe the effects of CypB knockdown on metastasis and invasion of SW1116 cells. Results The expression of CypB in colorectal cancer tissues with lymph node metastasis was significantly higher than that in colorectal cancer tissues without lymph node metastasis (P<0.01). Compared with SW1116-NC, the expression of CypB of SW1116-siCypB significantly reduced (P<0.01), and the performance of metastasis and invasion significantly decreased (P<0.01). Conclusion CypB plays an important role in lymph node metastasis of colorectal cancer, which makes it a potential target for the prevention and treatment of lymph node metastasis of colorectal cancer.

Key words: cyclophilin B, colorectal cancer, lymph node metastasis