Effects of levosimendan on kidney injury in ventricular fibrillation-induced cardiac arrest rats
2019, 39 (12):
Objective · To investigate the effects of levosimendan on kidney injury in the rat model of cardiopulmonary resuscitation. Methods · Twenty-five healthy adult male SD rats were randomly divided into three groups, which were sham group (S group, n5), levosimendan group (L group, n10) and control group (C group, n10). Cardiac arrest and cardiopulmonary resuscitation procedure were created in L group and C groupinducing ventricular fibrillation. L group was treated with levosimendan during and after resuscitation, while C group and S group were given equivalent volume of saline solution. S group was not induced into cardiac arrest and resuscitation. Serum interleukin-6 (IL-6), IL-10, tumor necrosis factor-α (TNF-α), creatinine (SCr), blood urea nitrogen (BUN) and cystatin-C (CysC) levels were compared between L group and C group at 1, 4 and 6 h after resuscitation.Three groups of rats were sacrificed, and the pathological changes of kidney tissues were observed at 6 h after resuscitation. Results · All rats were resuscitated successfully. No differences were found between the three groups about baseline data. After resuscitation, compared with S group, the levels of serum inflammatory cytokines and kidney function indicators increased dramatically (all PP0.000, P0.002, P0.036) than those in C group, and there were significant differences between the two groups (all P0.000). In resuscitation after 1, 4 and 6 h, the levels of SCr (P0.001, P0.007, P0.472), BUN (P0.001, P0.004, P0.122) and CysC (P0.493, P0.001, P0.175) were lower in L group than those in C group. Only 1 and 4 hours after resuscitation, the differences in the levels of SCr and BUN were significant, and only 4 hours after resuscitation, the difference in the level of CysC was significant between L group and C group. Both L and C group showed pathological characteristics of severe acute kidney injury, and the pathological injury scores of L group were alleviated compared with those of C group (all P0.000). Conclusion · Levosimendan can improve kidney injury of cardiac arrest and cardiopulmonary resuscitation model rats.
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