上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (5): 531-.

• 论著（基础研究） • 上一篇    下一篇

低剂量化疗联合树突状细胞生物免疫治疗对小鼠肺部肿瘤微环境的调节机制

钟 华, 钟润波, 韩宝惠   

  1. 上海交通大学 胸科医院肺内科, 上海 200030
  • 出版日期:2010-05-25 发布日期:2010-05-28
  • 通讯作者: 韩宝惠, 电子信箱: hbaohui@yahoo.com。
  • 作者简介:钟 华（1972—）, 女, 副主任医师, 博士；电子信箱: eddiedong8@hotmail.com。
  • 基金资助:

    上海交通大学附属胸科医院基金（Y209-03）

Mechanism of low-dose chemotherapy in combination with dendritic cell vaccines in modulation of murine lung cancer microenvironment

ZHONG Hua, ZHONG Run-bo, HAN Bao-hui   

  1. Department of Pulmonary Medicine, Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China
  • Online:2010-05-25 Published:2010-05-28
  • Supported by:

    Foundation from Chest Hospital Affiliated to Shanghai Jiaotong University, Y209-03

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摘要：

目的 探讨低剂量化疗联合树突状细胞（DC）生物免疫治疗作用于小鼠源性肺部肿瘤的机制。方法 小鼠皮下注射Lewis肺癌细胞（3LL），成瘤后分为对照组(不给予治疗)、低剂量化疗组（紫杉醇2 mg/kg）、DC免疫治疗组和联合治疗组（低剂量紫杉醇化疗联合DC生物免疫治疗），每组6只。第21天时，应用微渗析技术结合Luminex方法检测小鼠肿瘤内部和瘤旁组织中细胞因子干扰素诱导蛋白-10 （IP-10）、单核细胞趋化蛋白-1（MCP-1）、角质形成细胞（KC）和白介素-1α （IL-1α）水平的变化。结果 联合治疗组在部分时间点分泌的IP-10、MCP-1和KC水平显著高于其他三组（P<0.05）。各组瘤旁组织中，IP-10、MCP-1、KC和IL-1α的分泌水平差异均无统计学意义（P>0.05）。结论 低剂量化疗联合DC生物免疫治疗能有效地抑制小鼠肺部肿瘤的生长,其机制可能与调控肿瘤内部微环境有关。

关键词: 树突状细胞, 肺癌, 化疗调变

Abstract:

Objective To explore the mechanism of low-dose chemotherapy in combination with dendritic cell (DC) vaccine in treatment of murine lung cancer. Methods Lewis lung cancer cells (3LL) were inoculated subcutaneously in mice. Mice were divided into four groups (n=6) after tumor formation: control group (no treatment), low-dose chemotherapy group (treated with Paclitaxel 2 mg/kg), DC vaccine group and combined treatment group (treated with low-dose Paclitaxel and DC). On day 21, interferon-γ-induced protein 10 (IP-10), monocyte chemoattractant protein-1(MCP-1), keratinocyte (KC) and interleukin-1α (IL-1α) in intratumoral and peritumoral tissues were detected by microdialysis combined with Luminex technology in mice. Results Compared with the other three groups, the production of IP-10, MCP-1 and KC at some different time points was significantly higher in combined treatment group (P<0.05), while there was no significant difference in the production of IP-10, MCP-1, KC and IL-1α in peritumoral tissues among these four groups (P>0.05). Conclusion The combination of low-dose chemotherapy with DC vaccine may inhibit the growth of murine lung cancer,and the mechanism probably relates to regulation of microenviroment in the tumors.

Key words: dendritic cell, lung cancer, chemomodulation