上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

新疆汉族鼻咽癌患者HLA-A*02基因表达及多态性分析

刘 凯，谭 遥，李亚伟，王多明，吾甫尔江·艾克木，王若峥   

  1. 新疆医科大学附属肿瘤医院头颈放疗科， 乌鲁木齐 830011
  • 出版日期:2015-01-28 发布日期:2015-01-29
  • 作者简介:刘 凯（1989—）， 男， 硕士生; 电子信箱: 15199146547@126.com。
  • 基金资助:

    国家自然科学基金（81160327）；新疆维吾尔自治区国际合作基金（20126024；201141138）

Analysis of gene expression and polymorphism of allele HLA-A*02 of Han patients with nasopharyngeal carcinoma in Xinjiang

LIU Kai, TAN Yao, LI Ya-wei, WANG Duo-ming, WUFUERJIANG·Aikemu, WANG Ruo-zheng   

  1. Department of Head and Neck Radiation, the Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, China
  • Online:2015-01-28 Published:2015-01-29
  • Supported by:

    National Natural Science Foundation of China, 81160327; The Xinjiang International Collaboration Foundation of China, 20126024, 201141138

PDF (PC)

1035

摘要：

目的 探讨新疆汉族鼻咽癌患者HLA-A*02等位基因及其亚型的表达与鼻咽癌发病的关系。方法 采用聚合酶链反应/序列特异性引物（PCR-SSP）的方法对63例新疆汉族鼻咽癌患者和51名汉族健康志愿者HLA-A等位基因进行分析，比较2组HLA-A*02等位基因及其亚型的差异。结果 鼻咽癌患者共检出HLA-A*02等位基因51个（频率39.7%），健康人群检出HLA-A*02等位基因17个（频率16.7%），2组比较差异有统计学意义（P=0.002）；鼻咽癌患者共检出7个HLA-A*02等位基因亚型，健康人群检出4个等位基因亚型，2组比较差异无统计学意义（P>0.05），各亚型构成比比较差异无统计学意义（P>0.05）。结论 HLA-A*02等位基因与新疆汉族鼻咽癌患者的发病有关，新疆鼻咽癌患者HLA-A*02等位基因亚型分布存在自身特点。

关键词: 头颈部肿瘤, 鼻咽癌, HLA, 多态性, T细胞免疫

Abstract:

Objective To explore the relationship between the polymorphism of allele HLA-A*02 and the incidence of nasopharyngeal carcinoma (NPC) of Han patients in Xinjiang. Methods Polymerase chain reaction/sequence-specific primers (PCR-SSP) method was adopted to analyze HLA-A alleles of 63 Han patients with NPC in Xinjiang and 51 health Han volunteers. The differences of allele HLA-A*02 and its subtypes of two groups were compared. Results The frequency of allele HLAA*02 in NPC patients was 39.7% (51/126) and the frequency of allele HLA-A*02 in healthy people was 16.7% (17/102). The difference was statistically significant (P=0.002). Seven subtypes of allele HLA-A*02 were detected in NPC patients and 4 subtypes of allele HLA-A*02 were detected in healthy people. The difference was not statistically significant (P>0.05). The differences of constituent ratios of subtypes were not statistically significant (P>0.05). Conclusion The allele HLA-A*02 is relevant to the incidence of NPC of Han patients in Xinjiang. The distribution of subtypes of allele HLA-A*02 of NPC patients in Xinjiang have their own features.

Key words: head and neck cancer, nasopharyngeal carcinoma, HLA, polymorphism, T cell immunity