上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (7): 758-.

• 论著（基础研究） • 上一篇    下一篇

骨髓抑制—再生小鼠模型的建立及组织学改变的动态观察

卢俊钦, 孙文立, 毛文伟   

  1. 上海交通大学 药学院神经药理学实验室, 上海 200240
  • 出版日期:2010-07-25 发布日期:2010-07-26
  • 通讯作者: 毛文伟, 电子信箱: wwmao@sjtu.edu.cn。
  • 作者简介:卢俊钦（1990—）, 男, 口腔医学系2008级(七年制)学生；电子信箱: 461133087@qq.com。
  • 基金资助:

    国家自然科学基金（30570787）和上海交通大学本科生研究计划（T17015009）

Establishment of mouse bone marrow depression and regeneration model and dynamic observations of morphological changes

LU Jun-qin, SUN Wen-li, MAO Wen-wei   

  1. Laboratory of Neuropharmacology, School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
  • Online:2010-07-25 Published:2010-07-26
  • Supported by:

    National Natural Science Foundation of China, 30570787；Shanghai Jiaotong University Foundation of Participation in Research Program, T17015009

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摘要：

目的 动态观察骨髓抑制—再生模型小鼠的骨髓组织形态学变化并探讨其应用价值。方法 经尾静脉注射肿瘤化疗药物5-氟脲嘧啶（5-Fu）（250 mg/kg）诱导建立骨髓抑制-再生小鼠模型。分别于5-Fu注射前（对照组，n=6）及注射后第3、7、11、14天（模型3 d、7 d、11 d、14 d组，n=6）时，采集血样后分批处死小鼠，截取大腿骨，获取骨髓，一侧制备HE染色骨髓石蜡切片。动态观察骨髓抑制-再生过程中骨髓组织形态学变化特征。结果 对照组小鼠大腿骨髓腔充满大量单核细胞和红细胞，巨核细胞则散布于单核细胞和红细胞之间。在模型3 d组，单核细胞、红细胞和巨核细胞数量急剧减少；模型7 d组，上述细胞数量减少至最低。随后小鼠骨髓开始再生，模型11 d组可观察到骨髓腔内形成一种特征性区域性细胞分布，即骨髓腔下段大量红细胞集聚，中、上段则分别大量集聚单核细胞和巨核细胞；这种特征性细胞分布在模型14 d组开始消失。结论 在5-Fu诱发的小鼠骨髓抑制-再生过程中存在一种特征性细胞再聚集和再分散行为。

关键词: 5-氟脲嘧啶, 骨髓, 抑制, 再生, 组织形态学

Abstract:

Objective To dynamically observe the morphological changes of bone marrow of mouse bone marrow depression and regeneration model. Methods Mouse bone marrow depression and regeneration model was established by injection of 250 mg/kg 5-fluorouracil (5-Fu) via tail vein. Blood samples were taken before 5-Fu injection (control group, n=6) and 3 d, 7 d, 11 d and 14 d after 5-Fu injection (model 3 d group, model 7 d group, model 11 d group and model 14 d group, respectively, n=6), bone marrow samples were obtained after sacrifice of mice, and bone marrow paraffin sections were then prepared with HE staining. The morphological changes of bone marrow during bone marrow depression and regeneration were dynamically observed. Results Femur bone marrow cavity was saturated with large number of erythrocytes and mononuclear cells, and was sparsely decorated with megakaryocytes. The numbers of erythrocytes, mononuclear cells and megakaryocytes dramatically decreased in model 3 d group, and to a nadir in model 7 d group. Subsequently, bone marrow regeneration initiated, and repopulated cells formed a characteristically dynamic distribution in model 11 d group, with assembly of erythrocytes in lower part of femur and assembly of mononuclear cells and megakaryocytes in middle and upper part of femur, respectively. This distinguished feature began to disappear in model 14 d group. Conclusion There exist a characteristic cell assembly and disassembly process during mouse bone marrow depression and regeneration induced by 5-Fu.

Key words: 5-fluorouracil, bone marrow, depression, regeneration, morphology