上海交通大学学报(医学版)

• 论著(预防医学) • 上一篇    下一篇

青春前期十溴联苯醚暴露对雄性大鼠精子质量的影响

王 鹰,沙天宇,周静哲,陈丽梅,施 蓉,周义军   

  1. 上海交通大学  公共卫生学院, 上海 200025
  • 出版日期:2014-07-28 发布日期:2014-08-11
  • 通讯作者: 周义军, 电子信箱: zhouyj@shsmu.edu.cn。
  • 作者简介:王 鹰(1990—), 女, 09级预防医学本科生; 电子信箱: eagle_i@sjtu.edu.cn。
  • 基金资助:

    上海市科委自然基金(12ZR1416100);国家级大学生创新训练项目(201310248079);上海交通大学医学院基金(13XJ10005)

Effects of BDE-209 exposure on sperm quality of prepubertal male rats

WANG Ying, SHA Tian-yu, ZHOU Jing-zhe, CHEN Li-mei, SHI Rong, ZHOU Yi-jun   

  1. School of Public Health, Shanghai Jiao Tong University, Shanghai 200025, China
  • Online:2014-07-28 Published:2014-08-11
  • Supported by:
    Natural Science Foundation of Science and Technology Commission of Shanghai Municipality, 12ZR1416100; National Innovation Program for Undergraduates, 201310248079; Foundation of Shanghai Jiao Tong University School of Medicine, 13XJ10005

摘要:

目的 探讨青春前期十溴联苯醚(BDE-209)暴露对雄性大鼠精子质量的影响。方法 将24只3周龄雄性SD大鼠随机分为对照组和染毒组。于出生后第28日开始,染毒组分别给予2、10和50 mg/kg BDE-209(低、中、高剂量染毒组)灌胃染毒1个月,对照组给予纯玉米油灌胃1个月,于出生后第63日处死大鼠,观察精子形态学变化,计算精子畸形率,测定精子运动参数。结果 在染毒组可观察到香蕉形、无钩、胖头等典型的精子形态学改变。低、中、高剂量染毒组精子畸形率分别为(10.50±3.987)‰、(11.50±6.775)‰和(10.83±4.401)‰,对照组精子畸形率为(400±3688)‰,各染毒组精子畸形率均显著高于对照组,差异有统计学意义(P<0.05)。低、中、高剂量染毒组精子运动参数与对照组比较,差异均无统计学意义(P>0.05)。结论 BDE-209暴露对雄性SD大鼠的精子质量有一定的影响。

关键词: 十溴联苯醚, 精子, 大鼠, 生殖毒性

Abstract:

Objective To investigate the effects of BDE-209 exposure on the sperm quality of prepubertal male rats. Methods Twenty four 3-week old SD rats were randomly divided into the control group and three BDE-209 groups. BDE-209 groups were given BDE-209 of 2, 10, and 50 mg/kg by the intragastric injection for one month after 28 d of birth. The control group was given pure corn oil by the intragastric injection for one month. Rats were sacrificed on Day 63. The morphological changes of sperms were observed. The deformity rate of sperms was calculated and kinesis parameters were measured. Results Typical morphological changes such as banana-shaped, no hook, and fat head were observed in BDE-209 groups. The deformity rates of BDE-209 groups were (10.50±3.987)‰, (11.50±6.775)‰, and (10.83±4.401)‰, respectively. The deformity rate of control group was (4.00±3.688). The deformity rates of BDE-209 groups were significantly higher than that of the control group (P<0.05). The differences of kinesis parameters of BDE-209 groups and the control group were not statistically significant (P>0.05). Conclusion Exposure to BDE-209 has certain influence on the sperm quality of male SD rats.

Key words: BDE-209, sperm, rats, reproductive toxicity