上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

厄贝沙坦对5/6肾切除及腹主动脉缩窄大鼠左心室肥厚及心肌尿素转运蛋白B表达的影响

刘怡晟，陆玮，黄海东，刘爽，蒋更如   

  1. 上海交通大学 医学院附属新华医院肾内科， 上海 200092
  • 出版日期:2016-02-28 发布日期:2016-03-29
  • 通讯作者: 陆玮, 电子信箱: luweixh@126.com。
  • 作者简介:刘怡晟（1966—）， 男， 主治医师， 博士; 电子信箱: liuyisheng2002@sohu.com。
  • 基金资助:

    上海市卫生局资助项目(2010102)

Effects of irbesartan on left ventricular hypertrophy and expression of urea transporter B in myocardium of 5/6 nephrectomized rats with constricted abdominal aorta

LIU Yi-sheng, LU Wei, HUANG Hai-dong, LIU Shuang, JIANG Geng-ru   

  1. Renal Division, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2016-02-28 Published:2016-03-29
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2010102

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摘要：

目的 观察血管紧张素Ⅱ受体拮抗剂厄贝沙坦对5/6肾切除及腹主动脉缩窄大鼠左心室肥厚及心肌尿素转运蛋白B（UT-B）表达的影响。方法 SD大鼠随机分为假手术组、5/6肾切除组（肾衰组）、腹主动脉缩窄组（心衰组）、肾衰+心衰组及相应的厄贝沙坦干预组，每组8只。分组处理后，测定各组大鼠血清肌酐（SCr）、血尿素氮（BUN）、血管紧张素Ⅱ（AngⅡ）、B型利钠肽（BNP）；取大鼠心脏，测定左心室质量与全心质量的比值（LVW/HW）、全心质量与体质量的比值（HW/BW）、左心室质量与体质量的比值（LVW/BW）；RT-PCR和Western blotting检测心肌UT-B表达。结果 与假手术组比较，肾衰组、心衰组及肾衰+心衰组大鼠出现心肌肥厚，LVW/HW、HW/BW、LVW/BW增大，血清AngⅡ和BNP水平增高，心肌组织UT-B mRNA和UT-B蛋白表达上调；肾衰组和肾衰+心衰组SCr、BUN水平升高。与肾衰组、心衰组及肾衰+心衰组比较，相应的厄贝沙坦干预组大鼠的心肌肥厚明显减轻，LVW/HW、HW/BW、LVW/BW降低，血清AngⅡ和BNP下降，心肌UT-B mRNA和UT-B蛋白表达下调。与肾衰组和肾衰+心衰组比较，相应的厄贝沙坦干预组大鼠SCr、BUN水平降低。结论 5/6肾切除及腹主动脉缩窄均可引起大鼠心功能明显下降，出现左心室肥厚，同时心肌UT-B表达增加；厄贝沙坦在改善心、肾功能的同时抑制心肌UT-B表达，UT-B可能在肾功能不全合并心力衰竭的发生中具有重要作用。

关键词: 厄贝沙坦, 尿素转运蛋白B, 5/6肾切除, 腹主动脉缩窄, 大鼠

Abstract:

Objective To observe the effects of angiotensin Ⅱ receptor antagonist irbesartan on left ventricular hypertrophy and the expression of urea transporter B (UT-B) in myocardium of 5/6 nephrectomized rats with constricted abdominal aorta. Methods SD rats were randomly divided into the sham group, 5/6 nephrectomy group, abdominal aorta constriction group, 5/6 nephrectomy and abdominal aorta constriction group, and irbesartan group. Each group consisted of eight rats. Serum Cr, BUN, AngⅡ, and BNP were detected after rats were sacrificed. Rat hearts were harvested and the left ventricular weight/heart weight (LVW/HW), heart weight/body weight (HW/BW), and left ventricular weight/body weight (LVW/BW) were measured. The expression of UT-B in myocardium was determined by RT-PCR and Western blotting. Results Compared with the sham group, rats of 5/6 nephrectomy group, abdominal aorta constriction group, and 5/6 nephrectomy and abdominal aorta constriction group had the left ventricular hypertrophy and elevated LVW/HW, HW/BW, LVW/BW, serum AngⅡ, BNP, and mRNA and protein expressions of UT-B in myocardium. Levels of SCr and BUN of the 5/6 nephrectomy group and 5/6 nephrectomy and abdominal aorta constriction group increased. Compared with above three groups, rats of the irbesartan group had alleviated left ventricular hypertrophy and lower LVW/HW, HW/BW, LVW/BW, serum AngⅡ, BNP, and mRNA and protein expressions of UT-B in myocardium. Compared with the 5/6 nephrectomy group and 5/6 nephrectomy and abdominal aorta constriction group, levels of SCr and BUN of the irbesartan group were lower. Conclusion 5/6 nephrectomy and abdominal aorta constriction can cause significant decrease of heart function of rats, left ventricular hypertrophy, and elevated expression of UT-B. Irbesartan can improve heart and kidney functions and inhibit the expression of UT-B, which may play an important role in heart failure of rats with renal inadequacy.

Key words: irbesartan, urea transporter, 5/6 nephrectomy, constriction of the abdominal aorta, rat