上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (10): 1208-.doi: 10.3969/j.issn.1674-8115.2010.10.006

• 论著（基础研究） • 上一篇    下一篇

低氧培养对RL95-2细胞线粒体损伤及凋亡的影响

徐步芳1, 孙晓溪2, 冯 云1, 张爱军1, 程利南3   

  1. 1.上海交通大学 医学院附属瑞金医院生殖医学中心, 上海 200025； 2.中美合作上海集爱遗传与不育诊疗中心, 上海 200023； 3.复旦大学 上海医学院附属计划生育研究所, 上海 200032
  • 出版日期:2010-10-25 发布日期:2010-10-27
  • 通讯作者: 程利南, 电子信箱: linanc2@yahoo.com。
  • 作者简介:徐步芳（1977—）, 女, 博士生；电子信箱: ufang.xu@gmail.com。
  • 基金资助:

    上海交通大学医学院科技基金(YZ1041)

Effects of hypoxia culture on damage and apoptosis of RL95-2 cell line

XU Bu-fang1, SUN Xiao-xi2, |FENG Yun1, |ZHANG Ai-jun1, CHENG Li-nan3   

  1. 1.Reproduction Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China；2.Shanghai |JiAi Genetics and IVF Institute, Shanghai 200023, China；3.Institute of Family Planning, Shanghai Medical School, Fudan University, Shanghai 200032, China
  • Online:2010-10-25 Published:2010-10-27
  • Supported by:

    Science and Technology Foundation of Shanghai Jiaotong University School of Medicine, YZ1041

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摘要：

目的 探讨低氧培养对于人子宫内膜上皮腺癌细胞株（RL95-2）线粒体损伤及细胞凋亡的影响。方法 用含有不同浓度（0、10、30、62.5、125 μmol/L）氯化钴的培养液培养RL95-2细胞株。采用CCK-8法测定细胞增殖活性；JC-1荧光染色,流式细胞仪分析细胞线粒体膜电位变化；Annexin V/PI双染流式细胞仪检测细胞凋亡。结果 随着氯化钴浓度升高或作用时间延长(62.5 μmol/L作用下)， RL95-2细胞增殖活性逐渐下降，线粒体膜电位去极化增加，细胞凋亡率升高。结论 缺氧造成子宫内膜上皮细胞增殖活性下降，线粒体损伤，凋亡率升高。

关键词: 低氧培养, RL95-2细胞株, 线粒体损伤, 凋亡

Abstract:

Objective To investigate effects of hypoxia culture on mitochondria damage and apoptosis of human endometrial epithelial carcinoma cell line (RL95-2). Methods RL95-2 cells were cultured with medium containing different concentrations of CoCl2 (0, 10, 30, 62.5, and 125 μmol/L). Cell proliferation activity, mitochondria membrane potential, and apoptosis rate were determined via CCK-8 kit, JC-1 fluorescence staining with flow cytometry, and Annex V/PI. Results With CoCl2 concentration rising or extension of action time (62.5 μmol/L), the proliferation activity of RL95-2 cells was weak, while the depolarization of mitochondria membrane and the apoptosis rate were increased. Conclusion The condition of hypoxia induces decrease of proliferation activity, mitochondria damage, increase of apoptosis of endometrial epithelial cells.

Key words: hypoxia culture, RL95-2 cell line, mitochondria damage, apoptosis