上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (12): 1490-.doi: 10.3969/j.issn.1674-8115.2010.12.010

• 专题报道（创伤医学） • 上一篇    下一篇

兔瘢痕性喉气管狭窄模型的制备

杨希之, 毛小慧, 敖华飞, 孔德秋   

  1. 上海交通大学 医学院附属第三人民医院耳鼻咽喉头颈外科 创伤医学研究所, 上海 201900
  • 出版日期:2010-12-25 发布日期:2010-12-31
  • 通讯作者: 敖华飞, 电子信箱: david5882004@126.com。
  • 作者简介:杨希之（1983—）, 男, 硕士生；电子信箱: yxzh1002@yeah.net。

Establishment of rabbit model of cicatricial laryngotracheal stenosis

YANG Xi-zhi, MAO Xiao-hui, AO Hua-fei, KONG De-qiu   

  1. Department of Otolaryngology-Head and Neck Surgery, The Third People's Hospital, Institute of Traumatic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai 201900, |China
  • Online:2010-12-25 Published:2010-12-31

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摘要：

目的 建立一种简单、稳定的兔瘢痕性喉气管狭窄模型。方法 将20只新西兰白兔分为实验组（n=10）和对照组（n=10）。实验组横向切开兔气管，用尼龙刷进行气管黏膜刮擦后闭合气管；对照组切开兔气管后不进行任何处理，直接闭合气管。两组分别在第4、9、14和19天内窥镜观察气管瘢痕形成情况，并计算狭窄度；3周后处死动物，取喉部标本，HE染色后进行组织学观察。结果 经内窥镜观察，对照组动物气管未发现瘢痕组织增生；实验组动物气管均出现瘢痕组织增生，气管狭窄度随着时间延长而逐渐增加，刮擦后第19天气管狭窄度明显大于刮擦后第4天（P＜0.05）。组织学观察显示，实验组动物气管狭窄部位成纤维细胞增生，胶原纤维增厚。结论 兔瘢痕性喉气管狭窄模型制作方法简单易行，可用于喉气管狭窄治疗方法的研究。

关键词: 喉气管狭窄, 动物模型, 治疗

Abstract:

Objective To establish a simple and stable rabbit model of cicatricial laryngotracheal stenosis. Methods Twenty New Zealand rabbits were divided into experiment group (n=10) and control group (n=10).  Tracheas of rabbits in experiment group were annularly incised, and were closed after scraping of tracheal mucosa with a nylon brush. Tracheas of rabbits in control group were closed immediately after incision, without any special treatment. Formation of cicatrix of trachea was observed by endoscopy on day 4, 9, 14 and 19, and the degree of stenosis was calculated. Rabbits were sacrificed three weeks later, laryngopharyngeal samples were obtained, and microscopic observations were conducted with HE staining. Results Endoscopic observations revealed that there was no cicatricial tissue hyperplasia in rabbits of control group. However, cicatricial tissue hyperplasia occurred and increased in rabbits in experiment group in a time-dependent manner, and the degree of tracheal stenosis 19 d after scraping was more severe than that 4 d after scraping (P＜0.05). Proliferation of fibroblasts and thickening of collagen fibers in areas of tracheal stenosis were observed by microscopic observations. Conclusion The rabbit model of cicatricial laryngotracheal stenosis can be easily established, which can serve in the research of treatment of laryngotracheal stenosis.

Key words: laryngotracheal stenosis, animal model, therapy