上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (11): 1578-.doi: 10.3969/j.issn.1674-8115.2011.11.016

• 论著（基础研究） • 上一篇    下一篇

BDNF对脊神经根结扎大鼠脊髓背角星形胶质细胞活化的影响

汪 静1,2, 张 昕1, 杜冬萍1, 江 伟2   

  1. 上海交通大学附属第六人民医院 1.疼痛科, 2.麻醉科, 上海 200223
  • 出版日期:2011-11-28 发布日期:2011-11-29
  • 通讯作者: 杜冬萍, 电子信箱: dudp@sjtu.edu.cn。
  • 作者简介:汪 静（1986—）, 女, 硕士生；电子信箱: wang_xjq0521@163.com。

Effects of BDNF on activation of spinal dorsal horn astrocytes in rats with spinal nerve ligation

WANG Jing1,2, ZHANG Xin1, DU Dong-ping1, JIANG Wei2   

  1. 1.Pain Management Center, 2.Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2011-11-28 Published:2011-11-29

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摘要：

目的 观测脊神经根结扎（SNL）大鼠脊髓背角中脑源性神经营养因子（BDNF）与星形胶质细胞活化标记蛋白胶质细胞纤丝酸性蛋白（GFAP）表达的变化，以及BDNF清除剂TrkB/Fc对GFAP表达和疼痛的影响。方法 取18只SD大鼠，随机分为空白对照组、假手术组和SNL组(n=6)。分别于术前、术后第1~6天测定大鼠50%机械缩爪阈值（50% PWT），末次测定后取腰膨大处脊髓背角经Western blotting检测GFAP和BDNF的表达。另取18只SD大鼠，随机分为对照组、SNL+安慰剂组和SNL+TrkB/Fc组（n=6），其中对照组仅行鞘内置管术；SNL+安慰剂组和SNL+TrkB/Fc组分别在行SNL术前，予鞘内注入PBS或TrkB/Fc 10 μL，术后1次/d×6 d，每次注药前1 h测定50% PWT，末次给药后1 h取大鼠腰膨大处脊髓背角经Western blotting检测GFAP的表达。结果 术后第1~6天，SNL组手术同侧后肢50% PWT均较术前基础值显著下降（P<0.01）；术后第6天，SNL组手术同侧脊髓背角BDNF和GFAP表达均较空白对照组显著升高（P<0.01）；SNL+TrkB/Fc组大鼠手术同侧50% PWT与基础值比较无明显变化（P>0.05），手术同侧脊髓背角GFAP表达与对照组比较差异无统计学意义（P>0.05）。结论 BDNF活化的脊髓星形胶质细胞对SNL诱发的神经病理性疼痛具有调控作用。

关键词: 脑源性神经营养因子, 胶质细胞纤丝酸性蛋白, 星形胶质细胞, 神经病理性疼痛

Abstract:

Objective To observe the changes of expression of brain-derived neurotrophic factor (BNDF) and glial fibrillary acidic protein (GFAP) in rats with spinal nerve ligation (SNL), and investigate the effects of TrkB/Fc, BDNF inhibitor on expression of GFAP and pain. Methods Eighteen SD rats were randomly divided into blank control group, sham operation group and SNL group (n=6). Values of 50% paw withdrawal threshold (50% PWT) were measured before surgery and on six consecutive days after surgery. Spinal dorsal horn of the spinal enlargement was obtained after last measurement for determination of expression of GFAP and BDNF by Western blotting. Another 18 SD rats were randomly allocated into control group, SNL+placebo group and SNL+TrkB/Fc group (n=6). Rats in control group only underwent intrathecal catheterization, while rats in SNL+placebo group and SNL+TrkB/Fc group received intrathecal injection of either PBS or TrkB/Fc 10 μL once before the operation of SNL catheterization and 6 times after the operation. Values of 50% PWT were measured 1 h before drug administration. Spinal dorsal horn of the spinal enlargement was obtained 1 h after last injection for determination of the expression of GFAP by Western blotting. Results On the first to the sixth days after operation, values of 50% PWT on the ipsilateral hindpaw in SNL group were significantly lower than the baseline value before operation (P<0.01). On the sixth day after operation, the expression of BDNF and GFAP in the ipsilateral dorsal horn in SNL group was significantly higher than that in blank control group (P<0.01). However, the value of 50% PWT in the ipsilateral hindpaw in SNL+TrkB/Fc group was not significantly different from the baseline value (P>0.05), and the expression of GFAP in SNL+TrkB/Fc group was also not significantly different from that in control group (P>0.05). Conclusion BDNF-induced astrocyte activation contributes to the development of neuropathic pain produced by SNL.

Key words: brain-derived neurotrophic factor, glial fibrillary acidic protein, astrocyte, neuropathic pain