上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (1): 9-.doi: 10.3969/j.issn.1674-8115.2012.01.002

• 论著（基础研究） • 上一篇    下一篇

急性炎症状态下2型糖尿病大鼠的反应能力研究

韩亭亭1, 苏布德格日乐1, 胡耀敏1, 黄 融1, 易 静2   

  1. 上海交通大学 1.医学院附属仁济医院内分泌代谢病科, 上海 200127； 2.基础医学院细胞生物学教研室, 上海 200025
  • 出版日期:2012-01-28 发布日期:2012-01-29
  • 作者简介:韩亭亭（1986-）, 女, 硕士生；电子信箱: htt86226@163.com。
  • 基金资助:

    国家自然科学基金（81170758，30670988）；仁济医院科研培育基金 (RJPY10-004)；上海高校选拔培养优秀青年教师科研专项基金（jdy09018）

Responsive ability of rats with type 2 diabetes under acute inflammatory state

HAN Ting-ting1, SU Budegerile1, HU Yao-min1, HUANG Rong1, YI Jing2   

  1. 1.Department of Endocrinology and Metabolic Diseases, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China；2.Department of Cell Biology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2012-01-28 Published:2012-01-29
  • Supported by:

    National Natural Science Foundation of China, 81170758, 30670988；Foundation from Renji Hospital, RJPY10-004；Scientific Research Foundation for Excellent Young Teachers in Shanghai, jdy09018

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摘要：

目的 研究2型糖尿病大鼠和正常大鼠在急性炎症状态下的反应能力差异和血糖变化情况。方法 以2型糖尿病GK大鼠和Wistar大鼠（正常对照）作为实验对象，按5 mg/kg内毒素（LPS）腹腔注射构建大鼠脓毒症的危重病模型。观察LPS注射前后两种大鼠的一般情况，测定血清中促炎因子肿瘤坏死因子α（TNF-α）、白介素1β（IL-1β）和白介素6（IL-6）和抗炎因子白介素10（IL-10）的质量浓度，监测血糖的波动情况。结果 注射LPS后，Wistar大鼠的一般情况差，出现活动减少、嗜睡、竖毛怕冷、严重腹泻等症状；而GK大鼠一般状况良好，未出现上述表现。LPS注射前，GK大鼠血糖浓度显著高于Wistar大鼠；注射LPS后，两种大鼠血糖浓度均显著升高，GK大鼠血糖波动幅度小于Wistar大鼠，两种大鼠的峰值血糖浓度无明显差异。LPS注射前，GK大鼠血清中TNF-α和IL-6的质量浓度显著高于Wistar大鼠（P<0.01）；LPS注射后，两种大鼠血清中TNF-α、IL-1β、IL-6和IL-10的质量浓度均较LPS注射前显著升高（P<0.01），且Wistar大鼠血清中TNF-α、IL-1β和IL-10的质量浓度明显高于GK大鼠（P<0.01）。结论 与正常大鼠比较，2型糖尿病大鼠对于急性炎症刺激的耐受性更强。

关键词: 糖尿病, 脓毒症, 急性炎症, 血糖, 细胞因子

Abstract:

Objective To investigate the differences of responsive ability and blood glucose between rats with type 2 diabetes and normal rats under acute inflammatory state. Methods GK rats with type 2 diabetes and Wistar rats (normal controls) were selected, and critical ill models of sepsis were established by intraperitoneal injection of 5 mg/kg lipopolysaccharide (LPS). Before and after LPS injection, the general state was observed, serum mass concentrations of pro-inflammatory cytokines of tumor necrosis factor α (TNF-α), interleukin 1β(IL-1β) and interleukin 6 (IL-6) and anti-inflammatory cytokine of interleukin 10 (IL-10) were measured, and blood glucose fluctuation was monitored in Wistar rats and GK rats. Results After LPS injection, the general state of Wistar rats was poor, and symptoms such as reduced activity, drowsiness, hair erection and severe diarrhea emerged. However, GK rats stayed in good condition, and did not bear the above symptoms. Before LPS injection, the concentration of blood glucose of GK rats was significantly higher than that of Wistar rats. After LPS injection, the concentrations of blood glucose of Wistar rats and GK rats significantly increased, the range of blood glucose fluctuation of GK rats was smaller than that of Wistar rats, while there was no significant difference in the peak concentration of blood glucose between GK rats and Wistar rats. Before LPS injection, serum mass concentrations of TNF-α and IL-6 of GK rats were significantly higher than those of Wistar rats （P<0.01）. The serum mass concentrations of TNF-α, IL-1β, IL-6 and IL-10 after LPS injection were significantly higher than those before LPS injection in GK rats and Wistar rats （P<0.01）, and the mass concentrations of TNF-α, IL-1β and IL-10 in Wistar rats were significantly higher than those in GK rats after LPS injection （P<0.01）. Conclusion The tolerance to acute inflammation of rats with type 2 diabetes is higher than that of normal rats.

Key words: diabetes mellitus, sepsis, acute inflammation, blood glucose, cytokine