上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (4): 440-.doi: 10.3969/j.issn.1674-8115.2012.04.016

• 论著（基础研究） • 上一篇    下一篇

慢性应激大鼠脑源性营养因子的表达分析

王静华1, 肖泽萍1, 肖世富1, 彭素芳1, 董晓蔚2, 禹顺英1, 李华芳1, 李 霞1   

  1. 1.上海交通大学 医学院附属精神卫生中心, 上海 200030； 2.华东师范大学认知神经科学研究所, 上海 200062
  • 出版日期:2012-04-28 发布日期:2012-04-27
  • 通讯作者: 李 霞, 电子信箱: ja_1023@yahoo.com.cn。
  • 作者简介:王静华（1972—）, 女, 主治医师, 硕士；电子信箱: jinghuazm5@gmail.com。
  • 基金资助:

    国家自然科学基金（81101007）；上海市科委基金（09411965600）

Analysis of expression of brain-derived neurotrophic factor in rats with chronic unpredictable mild stress

WANG Jing-hua1, XIAO Ze-ping1, XIAO Shi-fu1, PENG Su-fang1, DONG Xiao-wei2, YU Shun-ying1, LI Hua-fang1, LI Xia1   

  1. 1.Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China；2.Institute of Cognitive Neuroscience, East China Normal University, Shanghai 200062, China
  • Online:2012-04-28 Published:2012-04-27
  • Supported by:

    National Natural Science Foundation of China, 81101007；Shanghai Science and Technology Committee Foundation, 09411965600

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摘要：

目的 探讨慢性不可预知轻度应激(CUMS)大鼠模型各脑区脑源性神经营养因子(BDNF) 的表达及抗抑郁药对BDNF表达的影响。方法 将24只雄性SD大鼠随机分为三组（n=8）：A组为对照组；B组为CUMS应激组；C组为CUMS应激+西太普兰用药组（每天腹腔注射西酞普兰水溶液2 mL，10 mg/kg）。实验为期6周，每周称量大鼠体质量，每3周测试大鼠的糖水偏爱度，造模前及造模6周末通过旷场试验评价大鼠行为。6周后处死大鼠获取脑组织，采用Real-Time PCR检测各脑区BDNF mRNA表达水平。结果 第6周末，B组大鼠的糖水偏爱度明显低于A、C两组（P＜0.05）且行为增多。应激实验开始后，B、C两组大鼠体质量均明显低于A组（P＜0.05）。B组BDNF mRNA在海马中的表达高于A组，但差异无统计学意义（P=0.07）；C组纹状体中BDNF mRNA表达明显低于A组（P＜0.05）。结论 慢性应激可能引起海马BDNF表达升高，抗抑郁药物可能导致纹状体BDNF表达降低。

关键词: 慢性不可预知轻度应激, 抑郁症, 脑源性神经营养因子

Abstract:

Objective To investigate the expression of brain-derived neurotrophic factor (BDNF) in different areas of brain in rats with chronic unpredictable mild stress (CUMS), and explore the effects of antidepressant on expression of BDNF. Methods Twenty-four male SD rats were randomly assighed to control group (group A), CUMS group (group B) and CUMS + citalpram group (intraperitoneal injection of citalpram 2 mL once a day, 10 mg/kg) (group C), with 8 rats in each group. The experiment lasted for 6 weeks. Body weight was measured each week, sugar preference was evaluated every 3 weeks, and behavior of rats was assessed through open field tests before model establishment and 6 weeks after model establishment. Animals were sacrificed after 6 weeks, brain tissues were obtained, and Real-Time PCR was employed to detect the expression of BDNF mRNA in different areas of brain. Results At the end of the sixth week, rats in group B had significantly lower sugar preference than group A and group C (P＜0.05), and had more behavior. After the beginning of stress experiment, the body weight of rats in group B and group C was significantly lower than that in group A (P＜0.05). The expression of BDNF mRNA in hippocampus of rats in group B was higher than that in group A, while there was no significant difference between them (P=0.07). The expression of BDNF mRNA in striatum of rats in group C was significantly lower than that in group A (P＜0.05). Conclusion Chronic stress may increase the expression of BDNF in hippocampus, while antidepressant may decrease the expression of BDNF in striatum.

Key words: chronic unpredictable mild stress, depression, brain-derived neurotrophic factor