上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

转化生长因子β1对人宫颈癌细胞上皮－间质转化的诱导作用

石玉荣1,2，陈素莲1,2，杨滢1，王慧2   

  1. 蚌埠医学院1.生物化学与分子生物学教研室， 2.检验诊断学实验中心， 蚌埠 233000
  • 出版日期:2015-04-28 发布日期:2015-04-29
  • 作者简介:石玉荣（1974—）， 女， 硕士， 副教授; 电子信箱: shiyr@126.com。
  • 基金资助:

    安徽省自然科学基金（KJ2014A162）

Effects of transforming growth factor β1 on inducing epithelial-mesenchymal transition of human cervical cancer cells

SHI Yu-rong1,2, CHEN Su-lian1,2, YANG Ying1, WANG Hui2   

  1. 1.Department of Biochemistry and Molecular Biology, 2.Research Center of Clinical Laboratory, Bengbu Medical Collage, Bengbu 233000, China
  • Online:2015-04-28 Published:2015-04-29
  • Supported by:

    Natural Science Foundation of Anhui Province, KJ2014A162

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摘要：

目的 探讨转化生长因子β1 （TGF-β1）对人宫颈癌HeLa细胞上皮-间质转化（EMT）发生的诱导作用。方法 用10 ng/mL的TGF-β1处理HeLa细胞72 h，电镜下观察细胞形态，real-time PCR和Western blotting检测EMT相关上皮标记分子和间质标记分子的表达，划痕试验、Transwell实验检测细胞侵袭和转移能力。结果 TGF-β1可诱导HeLa细胞呈现EMT细胞形态，并且下调上皮相关标记蛋白E-cadherin和β-catenin的表达，上调间质相关标记蛋白Vimentin和N-cadherin的表达；细胞的侵袭和转移能力明显增强。结论 TGF-β1能够诱导HeLa细胞发生EMT，促进细胞侵袭和转移。

关键词: 上皮－间质转化, 转化生长因子β1, 宫颈癌细胞, 侵袭, 转移

Abstract:

Objective To investigate the effects of transforming growth factor β1 (TGF-β1) on inducing the epithelial-mesenchymal transition (EMT) of HeLa cells of human cervical cancer. Methods HeLa cells were treated by TGF-β1 of 10 ng/mL for 72 h and the morphological characteristics of HeLa cell lines were observed under the electron microscope. The expressions of epithelial markers and mesenchymal markers relevant to EMT were detected by the real-time PCR and Western blotting. The invasion and migration of HeLa cells were detected by the wound healing assay and Transwell chambers. Results TGF-β1 could induce morphological alteration of HeLa cells from epithelial morphology to mesenchymal morphology, down-regulate the expressions of epithelial marker of E-cadherin and β-catenin, up-regulate the expressions of mesenchymal makers of Vimentin and N-cadherin, and significantly increase the invasion and migration of HeLa cells. Conclusion TGF-β1 can induce the incidence of EMT of HeLa cells and enhance the invasion and migration of HeLa cells.

Key words: epithelial-mesenchymal transition, transforming growth factor β1, cervical cancer cells, invasion, metastasis