上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

光化学组织黏附技术修复兔眼睑创口的实验研究

沈琼1，姚敏2，杨鹏高2   

  1. 上海交通大学 医学院附属第三人民医院 1.超声科， 2.烧伤整形科， 上海 201999
  • 出版日期:2015-08-28 发布日期:2015-09-30
  • 作者简介:沈琼（1981—），女，住院医师，学士; 电子信箱: shenqiong116@163.com。
  • 基金资助:

    上海市卫生局青年基金（20124Y148）；上海交通大学新华基金（12XJ22012）

Experimental study on repairing lacerations on rabbit eyelids by photochemical tissue bonding

SHEN Qiong1, YAO Min2, YANG Peng-gao2   

  1. 1.Department of Ultrasonic, 2.Department of Burn and Plastic Surgery, Shanghai Third Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China
  • Online:2015-08-28 Published:2015-09-30
  • Supported by:

    Shanghai Municipal Health Bureau Fund for Young Scientists, 20124Y148; Xinhua Fund of Shanghai Jiao Tong University, 12XJ22012

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摘要：

目的  探讨光化学组织黏附技术（PTB）在修复兔眼睑创口中的应用价值。方法  在兔眼上睑皮肤做横行、纵行和斜行（45°）皮肤全层切口，分别采用PTB技术（PTB组）和缝合（缝合组）封闭创口。PTB组照射剂量分别为25、50和100 J/cm2，辐射度均为0.25 W/cm2。以非处理和激光照射（100 J/cm2）创口作为对照组。分别测定泄露压和皮肤抗张力，观察术后第1、3和7日创口周围真皮内炎症细胞浸润情况。结果  PTB组各亚组（25、50和100 J/cm2，0.25 W/cm2）泄露压与缝合组的差异均无统计学意义（P>0.05），其各亚组间的差异亦无统计学意义（P>0.05）；术后1周内，PTB组创口皮肤抗张力明显高于对照组，而在1周后，各组间皮肤抗张力的差异均无统计学意义（P>0.05）。与缝合组比较，PTB组操作时间明显缩短（P<0.05）；术后第1日，PTB组创口周围真皮内炎症细胞浸润程度较轻（P<0.05）。结论  PTB技术在修复眼睑创口的应用中可达到与缝合相当的抗张力效果，且具有操作更简便和术后炎症反应轻微的优点。

关键词: 光化学组织黏附技术, 修复, 眼睑, 创口

Abstract:

Objective  To investigate the application value of photochemical tissue bonding (PTB) technique on repairing lacerations on rabbit eyelids. Methods  Parallel, perpendicular, and oblique (45°) incisions were made on rabbit upper eyelid skin and closured by PTB (PTB group) or suturing (suturing group). The exposure doses of PTB group were 25, 50, 100 J/cm2 and radiancy was 0.25 W/cm2. Incisions that were not treated or underwent laser exposure (100 J/cm2) served as controls. Leak pressure and skin strength were measured and inflammatory infiltration of surrounding dermis on 1, 3, and 7 d after operation were observed. Results  The differences of leak pressure between PTB subgroups (25, 50, 100 J/cm2, 0.25 W/cm2) and suturing group, and among  PTB subgroups were not statistically significant (P>0.05). The skin strength of PTB group was remarkably higher than that of the control group within one week after operation and the differences of skin strength among groups one week after operation were not statistically significant (P>0.05). Compared with the suturing group, operation time of the PTB group was significantly shorter (P<0.05) and the inflammatory infiltration of surrounding dermis on 1 d after operation was milder (P<0.05). Conclusion  PTB can achieve the same anti-tension effect as suturing for repairing the laceration on eyelids with simpler operation and milder inflammatory infiltration.

Key words: photochemical tissue bonding, repair, eyelid, laceration