上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

津力达颗粒改善1型糖尿病大鼠肝脏损伤的疗效及其可能机制

叶菲，卢晓晓，刘子毓，陈海燕，石勇铨，刘志民   

  1. 第二军医大学附属长征医院内分泌科， 上海 200003
  • 出版日期:2016-02-28 发布日期:2016-03-29
  • 作者简介:叶菲(1986—)， 女， 主治医师， 硕士; 电子信箱: y_aichacha@163.com。

Therapeutic effect of Jinlida granules on the liver injury of mice with type 1 diabetes mellitus and possible mechanisms

YE Fei, LU Xiao-xiao, LIU Zi-yu, CHEN Hai-yan, SHI Yong-quan, LIU Zhi-min   

  1. Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Online:2016-02-28 Published:2016-03-29

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摘要：

目的 探讨津力达颗粒对1型糖尿病大鼠肝脏损伤的疗效及可能机制。方法 采用链脲佐菌素腹腔一次性注射诱发SD大鼠糖尿病模型。将成模大鼠随机分为糖尿病模型组（DM组）、0.75 g/kg药物组、1.5 g/kg药物组、3.0 g/kg药物组，另设正常对照组（NC组），每组10只。给药8周后测定空腹血糖（FBG）、糖化血红蛋白(HbA1C)、胰岛素、C肽、肝功能指标、肝脏纤维化指标、氧化应激指标、炎症指标，观察肝组织病理学改变。结果 DM组与NC组比较，FBG、肝酶、肝脏纤维化指标、氧化应激指标、炎症因子水平均升高；3.0 g/kg药物组给药后谷丙转氨酶（GPT）、谷草转氨酶（GOT）、碱性磷酸酶（AKP）、透明质酸（HA）、Ⅳ型胶原（Ⅳ-C）、Ⅲ型前胶原（PC-Ⅲ）、血清层黏连蛋白（LN）水平降低，超氧化物歧化酶 （SOD）、丙二醛（MAD）、一氧化氮（NO）、活性氧簇（ROS）水平降低, 谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）水平升高；3个药物组给药后C反应蛋白（CRP）、白介素-6（IL-6）均显著降低，大鼠肝纤维化病变减轻。结论 津力达颗粒对1型糖尿病大鼠的肝损伤有保护作用，其机制可能与降低氧化应激反应以及改善炎症状态有关。

关键词: 津力达颗粒, 糖尿病, 肝损伤, 肝脏纤维化, 氧化应激, 炎症

Abstract:

Objective To investigate the therapeutic effect of Jinlida granules on the liver injury of mice with type 1 diabetes mellitus and possible mechanisms. Methods The SD mouse model of type 1 diabetes mellitus was established by a single intraperitoneal injection of streptozotocin (STZ). The model rats were randomly divided into the model group (DM group), 0.75 g/kg, 1.5 g/kg, and 3.0 g/kg therapy groups. A normal control group (NC group) was established and there were 10 rats in every group. The fasting glucose (FBG), HbA1C, insulin, C-peptide, liver function indexes, hepatic fibrosis indexes, oxidative stress indexes, and inflammation indexes were detected and pathological changes of liver tissues were observed 8 weeks after the administration. Results Compared with the NC group, levels of FBG, liver enzyme, hepatic fibrosis indexes, oxidative stress indexes, and inflammation factors of the DM group increased. Levels of GPT, GOT, AKP, HA, IV-C, PC-Ⅲ, LN, SOD, MAD, NO, and ROS of the 3.0 g/kg therapy group decreased after administration, while levels of GSH-PX and CAT increased. Levels of CRP and IL-6 of three therapy groups decreased significantly and the hepatic fibrosis alleviated. Conclusion Jinlida granules have protective effect on the liver injury of mice with type 1 diabetes mellitus. The mechanism may be relevant to the decrease of oxidative stress reaction and alleviation of inflammation.

Key words: Jinlida granules, diabetes mellitus, liver injury, hepatic fibrosis, oxidative stress； inflammation