上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (2): 138-.doi: 10.3969/j.issn.1674-8115.2018.02.004

• 论著（基础研究） • 上一篇    下一篇

3， 3’ - 二吲哚基甲烷对脂多糖诱导下牙周膜细胞分泌炎症因子的影响

周菊妹 1，苑克勇 2，林文珍 2，胡戌琛 2，晋巧巧 2，牛晨光 2   

  1. 1. 江苏省昆山市第三人民医院口腔科，昆山 215316；2. 上海交通大学 医学院附属第九人民医院牙体牙髓科，上海市口腔医学重点实验室，上海市口腔医学研究所，国家口腔疾病临床研究中心，上海 200011
  • 出版日期:2018-02-28 发布日期:2018-03-09
  • 通讯作者: 牛晨光，电子信箱：niuchg09@alumni.sjtu.edu.cn。
  • 作者简介:周菊妹（1974—），女，副主任医师，学士；电子信箱：dentist_zhoujm98@163.com。
  • 基金资助:
    国家自然科学基金（81570964）；上海市科学技术委员会基金（1714090350）

Influence of 3,3’-diindolylmethane on expression of inflammatory cytokines in periodontal ligament cells induced by lipopolysaccharide

ZHOU Ju-mei1, YUAN Ke-yong2, LIN Wen-zhen2, HU Xu-chen2, JIN Qiao-qiao2, NIU Chen-guang2   

  1. 1. Department of Stomatology, Kunshan Third People’s Hospital, Kunshan 215316, China; 2. Department of Endodontics, Shanghai Ninth People’s  Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai 200011, China
  • Online:2018-02-28 Published:2018-03-09
  • Supported by:
    National Natural Science Foundation of China, 81570964；Project of Shanghai Municipal Committee of Science and Technology, 1714090350

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摘要：

目的 · 探讨 3,3’- 二吲哚基甲烷（3,3’-diindolylmethane，DIM）对脂多糖（lipopolysaccharide，LPS）诱导的人牙周膜细胞
（human periodontal ligament cells，hPDLCs）分泌炎症因子的影响，并初步探讨其相关作用机制。方法 · 分离培养 hPDLCs，CCK-8 法
测定 DIM 对 hPDLCs 增殖的影响，检测 DIM 毒性浓度范围。将 hPDLCs 分为 4 组：空白组加入不含 LPS 和 DIM 的无血清 DMEM；
LPS 组仅加入含 LPS（终浓度为 10 μg/mL）的无血清 DMEM；低浓度组含 10 μg/mL LPS+6.25 μmol/L DIM；高浓度组含 10 μg/mL
LPS+12.50 μmol/L DIM。培养 12 h，酶联免疫吸附试验检测各组上清液中 TNF-α、IL-1β 和 IL-6 浓度，Western blotting 检测 hPDLCs
内丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）和核因子 κB（nuclear factor κB，NF-κB）信号通路中蛋白的变
化。结果 · DIM 在 50 μmol/L 范围内时细胞活力不受影响（P>0.05）。低浓度和高浓度 DIM 均抑制 LPS 刺激下 hPDLCs 分泌炎症因子
TNF-α、IL-1β 和 IL-6（P<0.05），抑制效果随 DIM 的浓度升高而增强。DIM 可显著抑制 LPS 激活 NF-κB 信号通路。结论 · DIM 可能
通过抑制 NF-κB 信号通路的激活，抑制 LPS 诱导的 hPDLCs 分泌促炎因子 TNF-α、IL-1β 和 IL-6。

关键词: 人牙周膜细胞；3,3&rsquo, - 二吲哚基甲烷；炎症因子；核因子 &kappa, B

Abstract:

Objective · To investigate the effect of 3,3’-diindolylmethane (DIM) on the expression of inflammatory cytokines in human periodontal ligament cells (hPDLCs) induced by lipopolysaccharide (LPS) and to study the related mechanism. Methods · hPDLCs were isolated and cultured, and
CCK-8 method was used to detect the effect of DIM on the proliferation of hPDLCs. hPDLCs were randomly divided into 4 groups: blank group (without
LPS and DIM), LPS group (10 μg/mL LPS), 10 μg/mL LPS+6.25 μg/mL DIM, 10 μg/mL LPS+12.50 μg/mL DIM. The cells of all groups were cultured
for 12 h. The protein levels of TNF-α, IL-1β and IL-6 in supernatant were detected by enzyme linked immunosorbent assay. The change of mitogenactivated protein kinase (MAPK) and nuclear factor κB (NF-κB) signaling pathways were detected by Western blotting. Results · The cell viability was
not affected when the DIM concentration was less than 50 μmol/L (P>0.05). DIM at 6.25 and 12.50 μg/mL reduced the LPS-induced expression of TNF-α,
IL-1β and IL-6 at protein levels (P<0.05). DIM inhibited the activation of the NF-κB signaling pathway. Conclusion · DIM can reduce the LPS-induced
inflammatory cytokine expression in hPDLCs via restraining the activation of the NF-κB signaling pathway.

Key words: human periodontal ligament cells, 3,3’-diindolylmethane, inflammatory cytokine, nuclear factor κB