上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (2): 133-.doi: 10.3969/j.issn.1674-8115.2018.02.003

• 论著（基础研究） • 上一篇    下一篇

青春期氰戊菊酯暴露对雄性大鼠睾丸组织氧化应激的影响

张晶晶，周义军，户宜，施蓉，张妍，田英，高宇   

  1. 上海交通大学医学院 公共卫生学院环境卫生学教研室，上海 200025
  • 出版日期:2018-02-28 发布日期:2018-03-09
  • 通讯作者: 高 宇，电子信箱：gaoyu_ciel@sjtu.edu.cn。
  • 作者简介:?张晶晶（1990—），女，硕士生；电子信箱：zhangjj_826@foxmail.com。
  • 基金资助:
    国家自然科学基金青年科学基金项目（81602823）；上海市卫生和计划生育委员会面上项目（201640174）；上海交通大学医学院附属新华医院“千天
    计划”（15QT08）；上海卫生计生系统重要薄弱学科建设计划（2016ZB0103）

Effects of fenvalerate exposure during puberty on oxidative stress in male rat testis

ZHANG Jing-jing, ZHOU Yi-jun, HU Yi, SHI Rong, ZHANG Yan, TIAN Ying, GAO Yu   

  1. Department of Environmental Health, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2018-02-28 Published:2018-03-09
  • Supported by:
    National Natural Science Foundation of China, 81602823; Scientific Research Program of Shanghai Municipal Commission of Health and Family Planning, 201640174; Early Life Plan funded by Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 15QT08; Developing Plan of Important Weak Disciplines from Shanghai Municipal Commission of Health and Family Planning, 2016ZB0103

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摘要： 目的 · 观察青春期氰戊菊酯暴露对雄性大鼠睾丸组织氧化应激的影响。方法 · 将 50 只 Sprague-Dawley（SD）雄性大鼠随机
分为对照（玉米油）组、低剂量（0.02 mg/kg 氰戊菊酯）组、中剂量（1 mg/kg 氰戊菊酯）组、高剂量（50 mg/kg 氰戊菊酯）组及干
预（50 mg/kg 氰戊菊酯 +100 mg/kg N- 乙酰基 -L- 半胱氨酸）组，每组 10 只，于 4 周龄开始连续染毒 2 个月。测定睾丸组织中丙二醛
（malondialdehyde，MDA）含量、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）和超氧化物歧化酶（superoxide dismutase，
SOD）活力，并观察大鼠睾丸组织形态结构。结果 · 与对照组相比，高剂量组大鼠睾丸组织中 MDA 含量升高，体质量、GSH-Px 和
SOD 活力均下降（均 P<0.05）；而干预组与高剂量组相比，MDA 含量降低，GSH-Px 活力升高（均 P<0.05）。睾丸组织形态学观察
发现，随着染毒剂量的增加，睾丸生精细胞排列疏松，细胞层数减少，曲细精管管腔内径增大。结论 · 青春期氰戊菊酯暴露可能导致
雄性大鼠睾丸组织氧化损伤。

关键词: 氰戊菊酯, 雄性大鼠, 睾丸, 氧化应激

Abstract:

Objective · To study the effects of fenvalerate exposure during puberty on oxidative stress in rat testis. Methods · Fifty male Sprague-Dawley
(SD) rats were randomly divided into the control group (corn oil), low dose group (0.02 mg/kg fenvalerate), moderate dose group (1 mg/kg fenvalerate),
high dose group (50 mg/kg fenvalerate) and intervention group (50 mg/kg fenvalerate+100 mg/kg N-acetyl-L-cysteine), ten rats for each group, for two
months by gavage at four weeks of age. Malondialdehyde (MDA) content, activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)
in testis and testicular tissue morphology were detected. Results · Compared with the control group, the rat body weight and activities of GSH-Px and
SOD in testis were significantly decreased in high dose group while MDA content was increased (all P<0.05). Compared with the high dose group, MDA
content was decreased and GSH-Px activity was increased in the intervention group (both P<0.05). The results of testicular histology showed that with
the increasing exposure dose, the spermatogenic cells were arranged loosely, the number of layers was decreased and the inner diameter of seminiferous
tubules was increased. Conclusion · Exposure to fenvalerate during puberty may induce oxidative damage in testis tissue of male rats.

Key words: fenvalerate, male rat, testis, oxidative stress