上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (2): 128-.doi: 10.3969/j.issn.1674-8115.2018.02.002

• 论著（基础研究） • 上一篇    下一篇

补骨脂素和异补骨脂素对人牙周膜细胞的抗炎作用

李笑甜 1, 2，周薇 2，宋忠臣 1, 2   

  1. 1. 上海交通大学 医学院附属第九人民医院牙周病科，上海 200011；2. 上海市口腔医学重点实验室 / 上海市口腔医学研究所，国家口腔疾病临床研究中心，上海 200011
  • 出版日期:2018-02-28 发布日期:2018-03-09
  • 通讯作者: 宋忠臣，电子信箱：szhongchen@sina.com。
  • 基金资助:
     国家自然科学基金（81670992，81271156）；上海交通大学医工交叉项目（YG2015MS04）

Anti-inflammation effects of psoralen and angelicin on human periodontal ligament cells in vitro

LI Xiao-tian1, 2, ZHOU Wei2, SONG Zhong-chen1, 2   

  1. 1. Department of Periodontology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 2. Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology, Shanghai 200011, China
  • Online:2018-02-28 Published:2018-03-09
  • Supported by:
    National Natural Science Foundation of China, 81670992, 81271156; Shanghai Jiao Tong University Medicine-Engineering Foundation, YG2015MS04

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摘要：

目的 · 观察补骨脂素和异补骨脂素对人牙周膜细胞炎症因子表达的影响。方法 · 组织块法原代培养人牙周膜细胞。CCK-8 试验检测 2 种药物对人牙周膜细胞活性的影响。用不同浓度的补骨脂素和异补骨脂素预处理人牙周膜细胞 2 h 后，再用牙龈卟啉单胞菌脂多糖（Porphyromonas gingivalis lipopolysaccharide，Pg-LPS）刺激，real-time PCR 和 ELISA 分别检测白介素 -1β（interleukin-1β，IL-1β）和白介素 -8（IL-8）的 mRNA 和蛋白表达。结果 · 采用组织块法可成功培养出人牙周膜细胞。12.5 μg/mL 及以下浓度的补骨脂素和异补骨脂素对人牙周膜细胞活性无影响。补骨脂素和异补骨脂素不仅可下调 Pg-LPS 诱导的人牙周膜细胞 IL-1β 和 IL-8 mRNA 的表达，还可抑制 IL-1β 和 IL-8 蛋白的分泌。结论 · 补骨脂素和异补骨脂素可显著抑制 Pg-LPS 诱导的人牙周膜细胞炎症因子的表达，可用于牙周炎的治疗和预防。

关键词: 人牙周膜细胞, 补骨脂素, 异补骨脂素, 抗炎

Abstract:

Objective · To investigate the effects of psoralen and angelicin on inflammation cytokine expression of human periodontal ligament cells (hPDLCs). Methods · hPDLCs were primarily cultured using tissue explant method. Effects of psoralen and angelicin on the cell viability were tested by CCK-8 assay. hPDLCs were stimulated by Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) after treatment with different concentrations of psoralen and angelicin for 2 h. mRNA expression of IL-1β and IL-8 were determined by real-time PCR. Enzyme-linked immunosorbent assay (ELISA) was used to measure the secretion of IL-1β and IL-8. Results · hPDLCs were cultured successfully by tissue explant method. Psoralen and angelicin (≤ 12.5 μg/mL) did not show significant effects on the cell viability of hPDLCs. Pg-LPS markedly elevated the mRNA expression of IL-1β and IL-8,which could be attenuated by psoralen and angelicin in a dose-dependent manner. Likewise, the up-regulated protein secretion of IL-1β and IL-8 could be significantly blocked by psoralen and angelicin. Conclusion · Psoralen and angelicin could attenuate the inflammatory response of hPDLCs induced by Pg-LPS. Therefore, psoralen and angelicin may act as natural agents to prevent and treat periodontitis.

Key words: human periodontal ligament cells, psoralen, angelicin, anti-inflammation