上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

Ras基因对人股动脉平滑肌细胞增殖过程中基因表达的影响

赵海光   

  1. 上海交通大学 医学院附属第九人民医院血管外科， 上海 200011
  • 出版日期:2013-09-28 发布日期:2013-09-29
  • 作者简介:赵海光（1977—），女，主治医师，博士; 电子信箱: sevilla877@gmail.com。
  • 基金资助:

    上海市卫生局面上项目（20114230）

Effect of Ras on gene expression profile during femoral artery smooth muscle cell proliferation

ZHAO Hai-guang   

  1. Department of Vascular Surgery, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2013-09-28 Published:2013-09-29
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 20114230

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摘要：

目的 观察Ras基因对人股动脉平滑肌细胞增殖过程中基因表达的影响。方法 以显性激活RAS基因和显性失活RAS基因重组腺病毒（AdRasV12和AdRasN17）转染人股动脉平滑肌细胞，采用Affymetrix基因芯片检测转染细胞的基因表达变化。结果 转染AdRasN17后，120个基因表达发生显著变化，其中43个基因表达上调，77个基因表达下调。转染AdRasV12后，231个基因被确定为差异表达，其中127个基因表达上调，104个基因表达下调。这些基因功能主要与细胞周期和凋亡、信号转导、代谢和对刺激应答有关。结论 激活和失活突变的Ras可改变人股动脉平滑肌细胞的基因表达，这些基因可能与动脉粥样硬化和再狭窄等心血管疾病发展过程中的动脉平滑肌细胞异常增殖有关。

关键词: 股动脉平滑肌细胞, RasN17, RasV12, 细胞增殖, 微阵列

Abstract:

Objective To investigate the effect of Ras on the gene expression during proliferation of human femoral artery smooth muscle cells. Methods Human femoral artery smooth muscle cells were transfected with recombinant adenovirus-mediated active and negative mutants of Ras gene (AdRasV12 and AdRasN17 respectively), and the gene expression profile was determined by Affymetrix GeneChip. Results After transfection with AdRasN17, 120 genes were identified with significantly altered expression, among which 43 genes were up-regulated and 77 genes were down-regulated. While in counterparts of AdRasV12, 231 genes were identified as differentially expressed, among which 127 genes were up-regulated and 104 were down-regulated. These genes were functionally categorized as cell cycle and apoptosis categories, signal transduction, metabolism and response to stimulus. Conclusion The active and negative mutants of Ras may alter the gene expression of human femoral artery smooth muscle cells, and these gene targets may be involved in abnormal arterial smooth muscle cell proliferation in cardiovascular diseases such as atherosclerosis and restenosis.

Key words: femoral artery smooth muscle cell, RasN17, RasV12, cell proliferation, microarray