上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

右美托咪定对缺血-再灌注大鼠肠黏膜屏障功能的影响

张 瑛，钟文晖，王爱忠   

  1. 上海交通大学附属第六人民医院麻醉科， 上海 201306
  • 出版日期:2014-04-28 发布日期:2014-05-13
  • 通讯作者: 王爱忠, 电子信箱: w19680420@sohu.com。
  • 作者简介:张 瑛（1980—）， 女， 主治医师， 硕士; 电子信箱: zhang198069ying@163.com。

Effects of dexmedetomidine on function of intestinal mucosal barrier of rats with ischemia-reperfusion injury

ZHANG Ying, ZHONG Wen-hui, WANG Ai-zhong   

  1. Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 201306, China
  • Online:2014-04-28 Published:2014-05-13

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摘要：

目的 观察右美托咪定对肠缺血-再灌注损伤（IRI）大鼠肠型脂肪酸结合蛋白（I-FABP）及炎症因子的影响，并探讨其作用机制。方法 建立大鼠肠IRI模型，随机分为假手术组、模型组、右美托咪定组。分别于缺血-再灌注后2 h和6 h时间点抽取大鼠腹主动脉血，采用酶联免疫吸附试验（ELISA）法检测大鼠血清I-FABP水平；于实验终点留取肠组织，ELISA法检测白介素-6（IL-6）和IL-10的含量，苏木精-伊红（HE）染色后经Chiu六级评分法判定大鼠小肠组织损伤程度。结果 与假手术组比较，模型组和右美托咪定组再灌注2 h和6 h时血清I-FABP水平明显升高，差异有统计学意义（P<0.05）；与模型组比较，右美托咪定组血清I-FABP含量显著下降，差异有统计学意义（P<0.05）。模型组和右美托咪定组肠组织中IL-6和IL-10的含量均明显高于假手术组，差异有统计学意义（P<0.05）；与模型组比较，右美托咪定组肠组织中IL-6含量降低，IL-10含量升高，差异均有统计学意义（P<0.05）。右美托咪定组Chiu评分显著低于模型组，但高于假手术组，差异均有统计学意义（P<0.05）。结论 右美托咪定可有效减少肠缺血-再灌注后炎症因子的产生，降低血浆中I-FABP水平，具有肠道保护作用。

关键词: 右美托咪定, 缺血-再灌注, 炎症因子, 肠型脂肪酸结合蛋白

Abstract:

Objective To observe the effects and to discuss the mechanism of dexmedetomidine on the intestinal fatty acid binding protein (I-FABP) and inflammatory factors of rats with intestinal ischemia-reperfusion injury. Methods The intestinal ischemia reperfusion injury model of rats was established and then rats were randomly divided into the sham operation group, ischemia-reperfusion group, and dexmedetomidine group. The aortic blood of rats was extracted at the 2 h and 6 h after ischemia-reperfusion. The plasma I-FABP levels were measured by ELISA. By the end of experiments, the contents of IL-6 and IL-10 in intestinal tissues were measured by ELISA. The intestinal tissues were stained by the HE and their injury severity was scored by the methods of Chiu's six levels. Results The plasma I-FABP levels of the ischemia-reperfusion group and dexmedetomidine group at 2 h and 6 h after reperfusion were significantly higher than those of the sham operation group. The differences were statistically significant (P<0.05). Compared to the ischemia-reperfusion group, the plasma I-FABP levels of the dexmedetomidine group were significantly decreased. The differences were statistically significant (P<0.05). The contents of IL-6 and IL-10 in intestinal tissues of the ischemia-reperfusion group and dexmedetomidine group were significantly higher than the sham operation group. The differences were statistically significant (P<0.05). Compared to the ischemia-reperfusion group, the contents of IL-6 in intestinal tissues of the dexmedetomidine group were lower, while the contents of IL-10 were higher. The differences were statistically significant (P<0.05). The Chiu's score of the dexmedetomidine group was significantly lower than that of the ischemia-reperfusion group but higher than that of the sham operation group. The differences were statistically significant (P<0.05). Conclusion Dexmedetomidine can effectively reduce the production of inflammatory factor after intestinal ischemia reperfusion, decrease the content of plasma I-FABP, and protect the intestinal functions.

Key words: dexmedetomidine, ischemia-reperfusion, inflammatory factor, intestinal fatty acid binding protein