上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

青春期前多溴联苯醚暴露对雄性大鼠睾丸标志酶活性的影响

沙天宇1，王 鹰1，周静哲1，陈丽梅1，周义军1,2，陈国元2   

  1. 1.上海交通大学 公共卫生学院， 上海 200025； 2.华中科技大学同济医学院公共卫生学院， 武汉 430030
  • 出版日期:2014-08-28 发布日期:2014-09-02
  • 作者简介:沙天宇（1991—）， 女， 2009级预防医学本科生; 电子信箱: shatianyu@sjtu.edu.cn。
  • 基金资助:

    上海市科委自然科学基金（12ZR1416100）；国家级大学生创新训练项目（201310248079）；上海交通大学医学院基金（13XJ10005）

Effects of prepubertal exposure to polybrominated diphenyl ethers on activity of enzymes in testis of male rats

SHA Tian-yu1, WANG Ying1, ZHOU Jing-zhe1, CHEN Li-mei1, ZHOU Yi-jun1,2, CHEN Guo-yuan2   

  1. 1.School of Public Health, Shanghai Jiao Tong University, Shanghai 200025, China; 2.School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China
  • Online:2014-08-28 Published:2014-09-02
  • Supported by:

    Natural Science Foundation of Science and Technology Commission of Shanghai Municipality, 12ZR1416100; National Innovation Program for Undergraduates of China, 201310248079； Foundation of Shanghai Jiao Tong University School of Medicine, 13XJ10005

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摘要：

目的 探讨青春期前十溴联苯醚（BDE-209）暴露对雄性大鼠睾丸标志酶活性的影响。方法 将24只雄性SD大鼠随机分为染毒组和对照组，染毒组再根据BDE-209浓度（2、10和50 mg/kg）分为低剂量组、中剂量组和高剂量组（以玉米油为溶剂），均于大鼠出生后第28日开始染毒，隔日1次，连续染毒30 d；对照组只给予玉米油。雄性SD大鼠于出生后第63日处死，计算睾丸和附睾的脏器系数，检测睾丸标志酶活性，并观察睾丸组织的病理学改变。结果 与对照组大鼠比较，经BDE-209染毒1个月后，各剂量染毒组大鼠的睾丸和附睾脏器系数无明显变化，差异无统计学意义（P>0.05）；睾丸标志酶酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)和γ谷氨酰转移酶(γ-GT)活性呈现下降趋势，但差异也无统计学意义（P>0.05）。各组大鼠的睾丸组织形态学均未观察到明显异常。结论 雄性SD大鼠青春期前BDE-209暴露对大鼠睾丸标志酶活性影响不明显。

关键词: 多溴联苯醚, 睾丸标志酶, 大鼠, 睾丸, 生殖毒性

Abstract:

Objective To investigate the effects of prepubertal exposure to BDE-209 on the activity of enzymes in testis of male rats. Methods Twenty-four male SD rats were randomly divided into the BDE-209 group and control group. The BDE-209 group was further divided into the low dose group, medium dose group, and high dose group (corn oil was used as the solvent) according to the concentration of BDE-209 (2, 10, and 50 mg/kg, respectively). Rats of the BDE-209 group were given BDE-209 every other day for 30 d after 28 d of birth. Rats of the control group were given corn oil. All male SD rats were executed on day 63 after birth. Organ coefficients of testis and epididymis were calculated. The activity of enzymes in testis was detected and pathological changes of the testicle tissue were observed. Results The organ coefficients of testis and epididymis of the BDE-209 group were not significantly different from those of the control group after being exposed to BDE-209 for one month. The differences were not statistically significant (P>0.05). The activity of acid phosphatases (ACP), alkaline phosphatase (AKP), lactate dehydrogenase (LDH), and gamma-glutamyl transpeptidase (γ-GT) decreased, but the differences were not statistically significant (P>0.05). No significant histomorphological changes of the testicle tissue of all groups were observed. Conclusion The effect of prepubertal exposure to BDE-209 on the activity of enzymes in testis of male SD rats is not significant.

Key words: poly brominated diphenyl ethers, enzymes in testis, rats, testis, reproduction toxicity