上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

蟾毒灵白蛋白纳米粒对结肠癌干细胞端粒酶活性的影响

祝利民1，沈克平1，张慧卿2，周 浩1，顾 贤1，潘传芳1，姚 琼1，郑佳露1，卢艳琳1   

  1. 1.上海中医药大学附属龙华医院肿瘤科， 上海 200032； 2.第二军医大学长海医院中医系， 上海 200433
  • 出版日期:2014-12-28 发布日期:2014-12-30
  • 作者简介:祝利民（1977—）， 女， 副主任医师， 硕士; 电子信箱: zhulimin2000@sina.com。
  • 基金资助:

    上海市教委预算内科研项目（2012JW33）

Effects of bufalin albumin nanoparticles on telomerase activity of stem cells of colon cancer

ZHU Li-min1, SHEN Ke-ping1, ZHANG Hui-qing2, ZHOU Hao1, GU Xian1, PAN Chuan-fang1, YAO Qiong1, ZHENG Jia-lu1, LU Yan-lin1   

  1. 1.Department of Oncology,Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China; 2.Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Online:2014-12-28 Published:2014-12-30
  • Supported by:

    Budget for Research Projects of Shanghai Municipal Education Committee, 2012JW33

PDF (PC)

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摘要：

目的 观察蟾毒灵白蛋白纳米粒对SW480结肠癌干细胞增殖及端粒酶活性的影响。方法 流式细胞仪分选SW480结肠癌细胞CD133+/CD44+亚群（SW480结肠癌干细胞），以不同浓度（1、10、50、100 nmol/L）的蟾毒灵及蟾毒灵白蛋白纳米粒进行干预，MTT法观察细胞增殖，采用端粒重复序列扩增－酶联免疫吸附测定法（TRAP-ELISA）检测结肠癌干细胞端粒酶活性的变化。结果 与对照组比较，低浓度（1、10 nmol/L）蟾毒灵及蟾毒灵白蛋白纳米粒干预对SW480结肠癌干细胞的增殖及端粒酶活性均无明显影响（P>0.05）；与对照组和中、高浓度（50、100 nmol/L）蟾毒灵组比较，相应浓度的蟾毒灵白蛋白纳米粒组SW480结肠癌干细胞的增殖及端粒酶活性明显受到抑制（P<0.05）。结论 50 nmol/L和100 nmol/L的蟾毒灵白蛋白纳米粒能显著抑制结肠癌干细胞的增殖和端粒酶活性，且效果优于蟾毒灵。

关键词: 结肠癌干细胞, 细胞增殖, 端粒酶活性, 蟾毒灵白蛋白纳米粒

Abstract:

Objective To observe the effects of bufalin albumin nanoparticles on the proliferation and telomerase activity of stem cells of colon cancer SW480. Methods The cell subset CD133+/CD44+ of colon cancer SW480 (stem cells of colon cancer SW480) were separated by the flow cytometry and intervened by different concentrations of bufalin and bufalin albumin nanoparticles (1, 10, 50, and 100 nmol/L). The MTT method was used to observe the proliferation of colon cancer stem cells and the TRAPELISA was adopted to detect the variations of telomerase activity. Results Compared to the control group, low concentrations (1 and 10 nmol/L) of bufalin and bufalin albumin nanoparticles had no significant effect on the proliferation and telomerase activity of stem cells of colon cancer SW480 (P>0.05). Compared to the control group and high concentrations (50 and 100 nmol/L) of bufalin, high concentrations (50 and 100 nmol/L) of bufalin albumin nanoparticles significantly inhibited the proliferation and telomerase activity of stem cells of colon cancer SW480 (P<0.05). Conclusion Bufalin albumin nanoparticles of 50 and 100 nmol/L can significantly inhibit the proliferation and telomerase activity of stem cells of colon cancer and the effects are better than those of bufalin.

Key words: colon cancer stem cells, cell proliferation, telomerase activity, bufalin albumin nanoparticles