上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

microRNA-24表达与黄曲霉毒素B1相关性肝细胞癌预后的关联性研究

龙满美1,2，黄小英3，姚金光3，刘懿霄1,2，马 韵1，夏 强2，龙喜带2,3   

  1. 1.广西医科大学第一附属医院病理科， 南宁 530021； 2.上海交通大学医学院附属仁济医院肝脏外科， 上海 200127； 3.右江民族医学院附属医院病理科， 百色 533000
  • 出版日期:2014-12-28 发布日期:2014-12-30
  • 通讯作者: 马 韵, 电子信箱: yunandama@hotmail.com; 龙喜带, 电子信箱: sjtulongxd@263.net。
  • 作者简介:龙满美(1989—)， 女， 硕士生; 电子信箱: longmanmei1989@163.com。
  • 基金资助:

    国家自然科学基金（81160255，81372639，81460423）；广西自然科学基金（2013GXNSFAA019251，2014GXNSFDA118021，2014GXNSFAA118144）；上海市曙光计划资助项目（13SG19）；上海市教委科研创新项目（13YZ035）

Correlation between microRNA-24 expression and prognosis of aflatoxin B1-related hepatocellular carcinoma prognosis

LONG Man-mei1,2, HUANG Xiao-ying2, YAO Jin-guang2, LIU Yi-xiao1,2, MA Yun1, XIA Qiang3, LONG Xi-dai2,3   

  1. 1.Department of Pathology, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China; 2.Department of Liver Surgery, the Affiliated Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 3.Department of Pathology, Youjiang Medical College for Nationalities, Baise 533000, China
  • Online:2014-12-28 Published:2014-12-30
  • Supported by:

    National Natural Science Foundation of China, 81160255, 81372639, 81460423; Guangxi Natural Science Foundation, 2013GXNSFAA019251, 2014GXNSFDA118021, 2014GXNSFAA118144; “Shu Guang” Project of Shanghai, 13SG19; Innovation Program of Shanghai Municipal Education Commission, 13YZ035

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摘要：

目的 探讨microRNA-24 (miR-24)表达对黄曲霉毒素B1 （AFB1）相关性肝细胞癌（HCC）预后的影响。方法 收集来自AFB1高暴露区TNM分期为Ⅰ~Ⅱ期的HCC患者（n=207）手术切除标本，通过TaqMan-PCR技术检测肿瘤组织中miR-24的表达情况，通过风险比例回归模型及逻辑回归模型分析miR-24表达与HCC预后及临床病理学特征的关系。结果 高miR-24表达影响HCC总体生存及肿瘤无复发生存（P<0.01），增加总体死亡风险及肿瘤复发风险分别为2.58倍和3.75倍，这种风险作用在有高AFB1暴露时更为明显，对应风险值分别为8.13（95％CI:4.46~14.84）和11.75（95％CI:5.15~26.79）。miR-24表达与HCC肿瘤大小、分化程度及血管密切相关（P<0.05），并调节AFB1-DNA加合物水平。结论 miR-24表达影响AFB1相关性HCC的预后，并可调节HCC临床病理特征。

关键词: miR-24, 肝细胞癌, 黄曲霉毒素B1, 预后

Abstract:

Objective To explore the effects of microRNA-24 (miR-24) expression on the prognosis of aflatoxin B1 (AFB1)-related hepatocellular carcinoma (HCC). Methods A total of 207 patients with Ⅰ-Ⅱ TNM-stage HCC from high AFB1 exposure areas were selected and the corresponding surgical resection samples were collected. Expression levels of MiR-24 in the tumor tissue samples were detected by the TaqMAN-PCR technique. Cox’s regression model and logistic regression model were adopted to analyze the relationship between the miR-24 expression and the prognosis and clinicopathological features of HCC. Results Elevated expression level of miR-24 affected overall survival and tumor recurrence-free survival (P<0.01). The overall death risk and tumor recurrence risk were increased by 2.58 times and 3.75 times. This risk effect was more noticeable under high AFB1 exposure condition and the corresponding risk values were 8.13 (95%CI: 4.46-14.84) and 11.75 (95%CI: 5.15-26.79), respectively. The expression level of miR-24 was closely correlated with primary tumor size, tumor differentiation, and micro-vessel density (P<0.05), and could regulate the level of AFB1-DNA adducts. Conclusion The miR-24 expression affects the prognosis of AFB1-related HCC and regulates the clinicopathological features of HCC.

Key words: microRNA-24, hepatocellular carcinoma, aflatoxin B1, prognosis