上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

姜黄素对自发性高血压大鼠血管重构的作用

谢 骏1,2，胡 珺2，毛家亮1   

  1. 1.上海交通大学 医学院附属仁济医院心血管内科， 上海 200127; 2.上海市徐汇区中心医院内科， 上海 200031
  • 出版日期:2014-12-28 发布日期:2014-12-30
  • 通讯作者: 毛家亮, 电子信箱: maojial@aliyun.com。
  • 作者简介:谢 骏（1979—）， 男， 主治医师， 学士; 电子信箱: xie.jun.79@outlook.com。

Effects of curcumin on vascular remodeling of rats with spontaneous hypertension

XIE Jun1,2, HU Jun2, MAO Jia-liang1   

  1. 1.Department of Cardiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 2.Department of Internal Medicine, Xuhui District Central Hospital, Shanghai 200031, China
  • Online:2014-12-28 Published:2014-12-30

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摘要：

目的 探讨姜黄素对自发性高血压大鼠血管重构的治疗作用。方法 42只雄性12周龄SHR大鼠，随机分为6组，每组7只;其中，5组为不同剂量姜黄素SHR组，每日分别予蒸馏水1 mL溶解的不同剂量姜黄素灌胃（剂量分别为25、50、100、200和400 mg/kg）;另1组为安慰剂SHR组，每日予蒸馏水1 mL灌胃。以7只同性别、周龄的WKy大鼠作为WKy对照组。实验开始时、实验6周后、实验12周后各测一次血压；12周后取大鼠颈动脉制成切片，行苏木精-伊红染色，测量颈动脉内径、中膜厚度、颈动脉中膜横截面积;采用免疫组织化学法，测量大鼠颈动脉中膜基质金属蛋白酶-9（MMP-9）的表达。结果 用药前、用药6周及用药12周，各姜黄素SHR组和安慰剂SHR组大鼠之间的收缩压、舒张压比较，差异无统计学意义（P>0.05），各姜黄素SHR组和安慰剂SHR组大鼠的收缩压、舒张压均高于WKy对照组大鼠（P<0.01）。400、200 mg/kg姜黄素SHR组颈动脉中膜厚度小于安慰剂SHR组（P<0.01），颈动脉内径与中膜厚度比值高于安慰剂SHR组（P<0.01）；100、50 mg/kg姜黄素SHR组颈动脉内径与中膜厚度比值高于安慰剂SHR组（P<0.05）；200 mg/kg姜黄素SHR组颈动脉内径大于安慰剂SHR组（P<0.05），颈动脉中膜横截面积小于安慰剂SHR组（P<0.05）。400 mg/kg姜黄素SHR组颈动脉中膜MMP-9表达高于安慰剂SHR组（P<0.05）。结论 姜黄素能在一定程度上减轻自发性高血压大鼠的颈动脉血管重构，上调颈动脉中膜MMP-9表达可能是作用途径之一。

关键词: 姜黄素, 自发性高血压, 血管重构, 基质金属蛋白酶-9, 大鼠

Abstract:

Objective To explore the therapeutic effect of curcumin on vascular remodeling of rats with spontaneous hypertension. Methods A total of 42 male SHR rats at 12 weeks of age were selected and randomly divided into six groups (n=7). Rats of five curcumin groups were given different doses (25, 50, 100, 200, and 400 mg/kg) of curcumin dissolved in l mL distilled water daily. Rats of one placebo group were given 1 mL distilled water daily. Another 7 WKy rats with same sex and age were used as the controls. Blood pressure of rats was measured before experiment and 6 and 12 weeks after experiment. Slices of the carotid artery of rats were stained by HE after 12 weeks. Media thickness (MT), lumen diameter (LD), and medial cross-sectional area (MSCA) of the carotid artery were measured. The expression of matrix metalloproteinase-9 (MMP-9) in the carotid artery was detected by immunohistochemical methods. Results The differences of systolic blood pressure (SBP) and diastolic blood pressure (DBP) of curcumin groups and placebo group before experiment and 6 and 12 weeks after experiment were not statistically significant (P>0.05). SBP and DBP of curcumin groups and placebo group were higher than those of the WKy control group (P<0.01). Compared to the placebo group, the MT of curcumin groups with doses of 400 and 200 mg/kg was thinner (P<0.01) and the LD/MT was higher (P<0.01); the LD/MT of curcumin groups with doses of 100 and 50 mg/kg was higher (P<0.05); the LD of curcumin group with doses of 200 mg/kg was larger (P<0.05) and MSCA was smaller (P<0.05); and the expression of MMP-9 of curcumin group with doses of 400 mg/kg was higher (P<0.05). Conclusion To some extent, curcumin can alleviate the carotid vascular remodeling of rats with spontaneous hypertension. The up-regulation of MMP-9 expression in carotid artery intima maybe one of the mechanisms.

Key words: curcumin, spontaneous hypertension, vascular remodeling, matrix metalloproteinase-9, rat