上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

系统性红斑狼疮肺出血患者血浆miRNAs的表达谱分析

涂洋，陈光亮，叶萍，陈晓翔   

  1. 上海交通大学 医学院附属仁济医院风湿科， 上海 200001
  • 出版日期:2015-08-28 发布日期:2015-09-30
  • 作者简介:涂洋（1988—）， 男， 硕士生; 电子信箱: tuyangzzu@126.com。
  • 基金资助:

    国家自然科学基金（81273306）

Analysis of expression profile of miRNAs in plasma of patients with systemic lupus erythematosus and diffuse pulmonary hemorrhage

TU Yang, CHEN Guang-liang, YE Ping, CHEN Xiao-xiang   

  1. Department of Rheumatology, RenJi Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China
  • Online:2015-08-28 Published:2015-09-30
  • Supported by:

    National Natural Science Foundation of China， 81273306

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摘要：

目的  筛选出系统性红斑狼疮肺出血患者血浆中异常表达的微RNA (miRNA)。方法  系统性红斑狼疮肺出血患者5例，健康对照组5例，将血浆标本分离提取miRNA用于芯片分析。在miRDB上预测miRNA的靶基因，采用PANTHER蛋白分类系统查找靶蛋白基因本体(GO) 功能分类。结果  与正常人比较，系统性红斑狼疮肺出血患者血浆中发现多个异常表达的miRNA，59个miRNA表达上调5倍，低于正常人表达水平30%以下的miRNA有15个。结论  许多miRNA在系统性红斑狼疮肺出血患者血浆中异常表达，提示可能存在一些miRNA可以用来预测系统性红斑狼疮肺出血患者的病情发展及预后。

关键词: 系统性红斑狼疮, 肺出血, 微RNA

Abstract:

Objective  To screen abnormally expressed microRNAs (miRNAs) in plasma of patients with systemic lupus erythematosus (SLE) and pulmonary diffuse alveolar hemorrhage (DAH). Methods miRNAs were isolated from plasma samples of five patients with SLE and DAH and five health controls for microarray analysis. The target genes of miRNAs were predicted by miRDB and the PANTHER protein classification system was used to find the gene ontology (GO) of target proteins. Results  Compared with controls, many abnormally expressed miRNAs were found in plasma of patients with SLE and DAH. Expression levels of 59 miRNAs up-regulated by 5 times and expression levels of 15 miRNAs were 30% lower than the normal expression level. Conclusion  Many miRNAs abnormally express in plasma of patients with SLE and DAH, which indicates that some miRNAs may be used to predict the development of disease and prognosis of patients with SLE and DAH.

Key words: systemic lupus erythematosus, diffuse alveolar hemorrhage, miRNAs