上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

炎症微环境下重组人釉原蛋白对人牙周膜成纤维细胞炎症因子表达的影响

廖蔚文，宋忠臣，束蓉，董家辰   

  1. 上海交通大学 医学院附属第九人民医院牙周病科 口腔医学院 上海市口腔医学重点实验室， 上海 200011
  • 出版日期:2016-01-28 发布日期:2016-02-26
  • 通讯作者: 束蓉, 电子信箱: shurong123@hotmail.com。
  • 作者简介:廖蔚文（1989—）， 女， 硕士生; 电子信箱: vivian050097@hotmail.com。
  • 基金资助:

    国家自然科学基金（81271156）

Effects of recombinant human amelogenin on expressions of inflammatory factors of human periodontal ligament fibroblasts under inflammatory microenvironment

LIAO Wei-wen, SONG Zhong-chen, SHU Rong, DONG Jia-chen   

  1. Department of Periodontology, College of Stomatology, Shanghai Key Lab of Stomatology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2016-01-28 Published:2016-02-26
  • Supported by:

    National Natural Science Foundation of China, 81271156。

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摘要：

目的 观察在炎症微环境下，重组人釉原蛋白（rhAm）对体外培养的人牙周膜成纤维细胞（hPDLCs）炎症因子表达的影响。方法 体外培养hPDLCs，采用免疫组织化学染色法鉴定hPDLCs。用10 μg/mL牙龈卟啉单胞菌（Porphyromonas gingivalis）脂多糖（LPS）模拟炎症微环境，选取20 μg/mL rhAm作用于hPDLCs，运用实时荧光定量PCR和ELISA技术检测炎症因子白介素（IL）-1β、IL-6和IL-8的表达。结果 免疫组织化学染色结果证实，培养细胞为hPDLCs。在所检测的4个时间点（6、12、24、72 h），10 μg/mL P. gingivalis LPS均可诱导IL-1β、IL-6和IL-8的表达；加入20 μg/mL rhAm后，各因子表达均有所降低。结论 rhAm可以抑制炎症微环境下hPDLCs炎症因子的表达。

关键词: 牙周膜成纤维细胞, 炎症微环境, 重组人釉原蛋白, 炎症因子

Abstract:

Objective To investigate the effects of recombinant human amelogenin (rhAm) on expressions of inflammatory factors of human periodontal ligament fibroblasts (hPDLCs) cultured in vitro under inflammatory microenvironment. Methods hPDLCs were cultured in vitro and identified by immunohistochemical staining method. The inflammatory microenvironment was simulated by lipopolysaccharide (LPS) with Porphyromonas gingivalis  of 10 μg/mL and hPDLCs were treated by rhAm of 20 μg/mL. Expressions of inflammatory factors interleukin-1β (IL-1β), IL-6, and IL-8 were detected by real-time PCR and ELISA. Results Results of immunohistochemical staining confirmed that cultured cells were hPDLCs. Expressions of IL-1β, IL-6, and IL-8 could be induced by LPS with P. gingivalis of 10 μg/mL at four time points (6, 12, 24, 72 h). Expressions of inflammatory factors decreased after being treated by rhAm of 20 μg/mL. Conclusion The rhAm can inhibit expressions of inflammatory factors of hPDLCs under inflammatory microenvironment.

Key words: periodontal ligament cell, inflammatory microenvironment, recombinant human amelogenin, inflammatory factor