上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

APLP2在子宫内膜癌中的表达及其对预后的预测价值

杨思勤1，何晓英1，田福举2，史蔚1，张健1   

  1. 上海交通大学 医学院附属国际和平妇幼保健院 1.妇科， 2.中心实验室， 上海 200030
  • 出版日期:2016-01-28 发布日期:2016-02-26
  • 作者简介:杨思勤（1974—）， 女， 主治医师, 学士; 电子信箱: ysq20060707@163.com。
  • 基金资助:

    国家自然科学基金青年项目(81401218)

Expression of APLP2 of patients with endometrial cancer and its value for predicting the prognosis

YANG Si-qin1, HE Xiao-ying1, TIAN Fu-ju2, SHI Wei1, ZHANG Jian1   

  1. 1.Department of Gynecology， 2.Department of Central Laboratory, International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2016-01-28 Published:2016-02-26
  • Supported by:

    National Natural Science Foundation of China， 81401218。

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摘要：

目的 探讨子宫内膜癌中淀粉样肽前体样蛋白2（APLP2）的表达，分析其与各临床参数的相关性。方法 通过数据挖掘，分析335例肿瘤基因图谱 (TCGA) 数据库中的子宫内膜癌RNA-SEQ数据并结合患者的完整生存期信息，采用Kaplan-Meier法分析APLP2表达与子宫内膜癌患者生存之间的关系。采用real-time PCR技术检测正常子宫内膜和子宫内膜癌各30例冰冻组织中APLP2 mRNA的表达情况。应用免疫组织化学法检测88例子宫内膜癌组织和32例正常子宫内膜组织中APLP2蛋白的表达。结果 Kaplan-Meier生存曲线分析表明，APLP2低表达的子宫内膜癌患者整体生存率明显降低（HR=2.87，P=0.002）。实时荧光定量PCR检测结果提示，APLP2 mRNA在子宫内膜癌组织中表达明显低于正常内膜组织（P=0.031）。免疫组织化学结果显示：APLP2蛋白在子宫内膜癌组织中的阳性表达率明显低于正常子宫内膜组织（P=0.025），其表达水平与雌激素受体表达水平相关（P=0.010）。结论 APLP2在子宫内膜癌中起到抑癌基因的功能。APLP2基因可能通过雌激素受体途径起作用。APLP2可能成为子宫内膜癌预后预测及治疗的靶点。

关键词: 淀粉样肽前体样蛋白2, 子宫内膜癌, 数据挖掘, 实时荧光定量PCR, 免疫组织化学

Abstract:

Objective To investigate the expression of amyloid precursor-like protein 2 (APLP2) in tissues of endometrial cancer and analyze the correlation between the expression of APLP2 and relevant clinical indexes. Methods RNA-SEQ data of 335 cases of endometrial cancer in the Cancer Genome Atlas (TCGA) database was analyzed by data mining. The correlation between the expression of APLP2 and the survival of patients with endometrial cancer was analyzed by Kaplan-Meier method based on RNA-SEQ data and complete survival information of patients. The mRNA expression of APLP2 in 30 frozen tissues of normal endometrium and 30 frozen tissues of endometrial cancer was detected by real-time PCR. The protein expression of APLP2 in 32 tissues of normal endometrium and 88 tissues of endometrial cancer was detected by immunohistochemical method. Results Analysis of Kaplan-Meier survival curve showed that the overall survival of patients with low expression of APLP2 significantly decreased (HR=2.87, P=0.002). Results of real-time PCR indicated that mRNA expressions of APLP2 in tissues of endometrial cancer were significantly lower than those in tissues of normal endometrium (P=0.031). Results of immunohistochemical method showed that the positive expression rate of APLP2 in tissues of endometrial cancer was significantly lower than that of in tissues of normal endometrium (P=0.025) and the expression level of APLP2 was correlated with the expression level of estrogen receptor (P=0.010). Conclusion APLP2 plays the role of tumor-suppressor gene for patients with endometrial cancer. APLP2 genes may take effect via the estrogen receptor and may be a target for the treatment and predicting the prognosis of patients with endometrial cancer.

Key words: amyloid precursor-like protein 2, endometrial cancer, data mining, real-time quantitative PCR, immunohistochemistry