上海交通大学学报(医学版)

• 论著(基础研究) • 上一篇    下一篇

dCTP焦磷酸酶在胃癌组织中的表达和分布及其临床相关性研究

徐苓1,2,夏立亮3,4,宋飞飞1,季萍1,王树军1,张勇1,沈立松2,王颖1,4   

  1. 1.上海交通大学 医学院 上海市免疫学研究所,上海 200025; 2.上海交通大学  医学院附属新华医院检验科,上海 200092;3.复旦大学 生命科学学院微生物学和微生物工程系,上海 200433;4.上海市疾病与健康基因组重点实验室 上海人类基因组研究中心,上海 201203
  • 出版日期:2016-02-28 发布日期:2016-03-29
  • 通讯作者: 王颖, 电子信箱: ywang@sibs.ac.cn。
  • 作者简介:徐苓(1985—), 女, 检验师, 学士; 电子信箱: immortality0018@aliyun.com。
  • 基金资助:

    上海市教委创新重点项目(13ZZ083);上海市自然科学基金(14ZR1436300);上海市卫计委项目(20114195)

Expression and distribution of dCTP pyrophosphatase 1 in gastric cancer tissues and its clinical relevance

XU Ling1,2, XIA Li-liang3,4, SONG Fei-fei1, JI Ping1, WANG Shu-jun1, ZHANG Yong1, SHEN Li-song2, WANG Ying1,4   

  1. 1.Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai200025, China; 2.Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3.Department of Microbiology and Microbial Engineering, School of Life Science, Fudan University, Shanghai 200433, China; 4.Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China
  • Online:2016-02-28 Published:2016-03-29
  • Supported by:

    Innovation Key Program of Shanghai Municipal Education Committee, 13ZZ083; Natural Science Foundation of Shanghai, 14ZR1436300; Foundation of Shanghai Municipal Commission of Health and Family Planning, 20114195

摘要:

目的 检测dCTP焦磷酸酶(DCTPP1)在胃癌组织中的表达和分布,分析其与临床胃癌病理特征的相关性,探讨DCTPP1参与胃癌发生与发展的可能机制。方法 收集胃癌手术患者正常、癌旁和肿瘤组织,Western blotting比较DCTPP1蛋白在3类组织中的表达,免疫组织化学法检测DCTPP1在胃癌肿瘤细胞中的表达和分布,探讨其与胃癌临床病理特征的相关性,分析DCTPP1在不同分期胃癌中的表达和分布。结果 来自于同一患者的3类组织样本中,胃癌组织中DCTPP1的表达量明显高于同一个体正常组织和癌旁组织。组织学观察结果显示,DCTPP1在胃癌组织细胞的细胞质及细胞核中均有表达,并且胃癌分期越高,其在细胞核中的表达越多。在伴随淋巴结转移的胃癌样本中,DCTPP1的细胞核表达高于未发生转移的肿瘤样本。结论 与正常和癌旁组织相比,DCTPP1在胃癌组织呈现高表达现象,并且其在肿瘤细胞核的富集表达与肿瘤的恶性程度和转移存在一定的统计学相关性。DCTPP1参与调节细胞内异常核苷酸的代谢,其在肿瘤细胞中的高表达和在细胞核中的聚集可能在胃癌的发生与发展过程中发挥重要作用。

关键词: 胃癌, dCTP焦磷酸酶, 病理相关性, 淋巴结转移

Abstract:

Objective To detect the expression and distribution of dCTP pyrophosphatase 1 (DCTPP1) in gastric cancer tissues, analyze the correlation between DCTPP1 and clinicopathological characteristics of gastric cancer, and explore possible mechanisms of the involvement of DCTPP1 in the occurrence and development of gastric cancer. Methods The normal, adjacent, and tumor tissues of patients with gastric cancer were collected. The expression of DCTPP1 in these tissues was compared by Western blotting. The distribution of expression of DCTPP1 in gastric cancer cells was detected by immunohistochemical method and its correlation with clinical pathological characteristics of gastric cancer was explored. The expression and distribution of DCTPP1 of gastric cancer with different stages were analyzed. Results For three types of tissues of the same patient, the expression of DCTPP1 in gastric cancer tissue was significantly higher than that of normal and adjacent tissues. Results of the histological observation indicated that DCTPP1 expressed in cytoplasm and nucleus of gastric cancer tissue cells. The expression of DCTPP1 in nucleus increased with the TNM stage. The expression of DCTPP1 in nucleus of gastric cancer tissues of patients with lymph node metastasis was higher than that of patients without lymph node metastasis. Conclusion Compared with normal and adjacent tissues, the expression of DCTPP1 in gastric cancer tissues is higher. The high expression of DCTPP1 in nucleus of gastric cancer cells statistically correlates with the severity and metastasis of tumors to a certain extent. Since DCTPP1 participates in regulating the metabolism of abnormal nucleotides, its high expression in tumor cells and the accumulation in nucleus may play an important role in the occurrence and development of gastric cancer.

Key words: gastric cancer, DCTPP1, pathological relevance, lymph node metastasis