上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

维生素D对羊膜腔注射脂多糖导致的新生鼠肺泡化阻滞的作用

刘成博1，杨奕辉2，李文1，张拥军1   

  1. 1.上海交通大学 医学院附属新华医院新生儿科， 上海 200092； 2.上海市嘉定区中心医院儿科， 上海 201800
  • 出版日期:2016-07-28 发布日期:2016-08-31
  • 通讯作者: 张拥军, 电子信箱: dryongjunzhang@163.com。
  • 作者简介:刘成博（1990—），女，硕士生; 电子信箱: liuchengbo90@126.com。
  • 基金资助:

     国家自然科学基金（81270729）

Effects of Vitamin D on intra-amniotic lipopolysaccharides-induced alveolarization arrest in neonatal rats

LIU Cheng-bo1, YANG Yi-hui2, LI Wen1, ZHANG Yong-jun1   

  1. 1.Department of Neonatology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2.Department of Pediatrics, Jiading Distinct Central Hospital, Shanghai 201800, China
  • Online:2016-07-28 Published:2016-08-31
  • Supported by:

    National Natural Science Foundation of China， 81270729

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摘要：

目的 探讨维生素D（VitD）对宫内绒毛膜羊膜炎诱发的肺泡化阻滞的改善作用及可能的机制。方法 通过孕鼠羊膜腔注射脂多糖（LPS）建立新生鼠支气管肺发育不良（BPD）模型。分为Saline组、LPS+Saline组、LPS+VitD（L）组和LPS+VitD（H）组。VitD干预的2组新生鼠出生后每日腹腔注射0.5和3 ng/g的1, 25(OH)2D3，Saline组和LPS+Saline组注射等量生理盐水。取第1、3、7日的新生鼠肺组织，通过苏木精-伊红（H-E）染色观察病理学改变。采用real-time PCR检测IL-1β和IFN-γ mRNA表达。结果 不同剂量的VitD均显著提高新生鼠出生率（均P=0.003）。出生后7 d，VitD干预的2组较LPS+Saline组肺泡计数显著增多（P=0.001，P=0.000），平均内衬间隔显著减少（均P =0.000），且VitD干预的2组IL-1β和IFN-γ mRNA表达显著低于LPS+Saline组（均P=0.000）。结论 予以VitD可降低新生鼠肺部IL-1β和IFN-γ表达，减轻BPD病理学改变。

关键词: 维生素D, 支气管肺发育不良, 炎症

Abstract:

Objective To investigate the role of Vitamin D (VitD) on amelioration of chorioamnionitis-induced alveolarization arrest and possible mechanisms. Methods A neonatal rat bronchopulmonary dysplasia (BPD) model was constructed by intra-amniotic injection of lipopolysaccharides (LPS) in pregnant rats. Rats were randomly assigned to the Saline group, LPS+Saline group，LPS+VitD (L) group, and LPS+VitD (H) group. Neonatal rats in the LPS+VitD (L) group and the LPS+VitD (H) group were intraperitoneally injected with 0.5 and 3 ng/g 1, 25(OH)2D3 respectively once a day for 7 d. Meanwhile, neonatal rats in the Saline group and the LPS+Saline group were intraperitoneally injected with the same volume of normal saline. The pulmonary tissues of neonatal rats were harvested 1, 3, and 7 d after the final injection and were stained by H-E staining for observing pathological changes. The mRNA expressions of IL-1β and IFN-γ  were detected using real-time PCR. Results Different concentrations of VitD could improve the birth rate of neonatal rats (P=0.003). The LPS+VitD (L) group and the LPS+VitD (H) group had significantly higher alveolar counts (P=0.001, P=0.000), remarkably decreased mean liner intercept (P=0.000), and significantly decreased mRNA expressions of IL-1β and IFN-γ as compared with the LPS+Saline group 7 d after birth (P=0.000). Conclusion Administration of VitD can significantly decrease the expressions of IL-1β and IFN-γ in lungs of neonatal rats and alleviate the pathological changes of BPD.

Key words: Vitamin D, bronchopulmonary dysplasia, inflammation