上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

泛耐药肺炎克雷伯菌噬菌体SH-Kp152234的生物学特性和基因组学研究

支烨 1, 2，罗婷婷 1，王睿 1，冯希佳 1，刘珂 1，石臣坤 1，王璐璐 1，胡付品 3，何平 1   

  1. 1.上海交通大学基础医学院免疫学与微生物学系，上海 200025；2. 优仕美地医疗，上海 201204；3. 复旦大学附属华山医院抗生素研究所，上海 200040
  • 出版日期:2017-03-28 发布日期:2017-03-30
  • 通讯作者: 何平，电子信箱：hpatsh@sjtu.edu.cn。胡付品，电子信箱：hufupin@163.com。
  • 作者简介:支烨（1985—），女，主管药师，硕士生；电子信箱：387889985@qq.com。
  • 基金资助:

    国家自然科学基金（81471908）

Characterization and genomic analysis of bacteriophage SH-Kp152234 infecting extensively drug-resistant Klebsiella pneumonia

ZHI Ye1, 2, LUO Ting-ting1, WANG Rui1, FENG Xi-jia1, LIU Ke1, SHI Chen-kun1, WANG Lu-lu1, HU Fu-pin3, HE Ping1   

  1. 1. Department of Microbiology and Immunology, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025, China; 2.Yosemite Clinic, Shanghai 201204, China; 3. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Online:2017-03-28 Published:2017-03-30
  • Supported by:

    National Natural Science Foundation of China, 81471908

PDF (PC)

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摘要：

目的 ·针对泛耐药肺炎克雷伯菌寻找宽宿主谱噬菌体，并对其生物学特性、基因组成进行分析。方法 ·以泛耐药肺炎克雷伯菌为宿主菌，从医院污水中分离纯化得到针对宿主菌的泛耐药肺炎克雷伯菌噬菌体。电子显微镜观察噬菌体的大小和形态，分析其效价、宿主谱、酸碱稳定性和热稳定性。抽取噬菌体DNA进行测序及分析。结果 ·分离到l株肺炎克雷伯菌噬菌体，命名为SH-Kp152234。电子显微镜观察显示其属短尾噬菌体，基因组为线状双链DNA，全长为40 578 bp，GC含量为52.85％。预测共有49个开放阅读框，保守序列具有已知的相关功能。结论 ·筛选得到肺炎克雷伯菌噬菌体SH-Kp152234的宿主谱较宽，潜伏期较短，可适应不同的温度和pH值，为治疗泛耐药肺炎克雷伯菌感染提供潜在依据。

关键词: 肺炎克雷伯菌, 噬菌体, 生物学特性, 基因组学

Abstract:

Objective · To identify broad-spectrum bacteriophages against extensively drug-resistant Klebsiella pneumonia and analyze their characteristics by biological and genomic methods. Methods · Multi-drug resistant Klebsiella pneumonia strains collected from a hospital were used as host bacteria to isolate and purify broad-spectrum phages in the wastewater at the same hospital area. The size and shape of phages were observed by transmission electron microscope. Titer, host range, pH stability and thermal stability were measured. Moreover, the DNA extracted from the phage SH-Kp152234 was sequenced and analyzed. Results · One strain of bacteriophage against Klebsiella pneumonia was isolated and named as SH-Kp152234. The electron microscope revealed it belongs to Podoviridae family. Moreover, genome of SH-Kp152234 showed to be a linear double-stranded DNA of 40 578 bp with the GC content of 52.85%. It was predicted to have 49 open reading frames with related known functions. Conclusion · SH-Kp152234, with a broad host range and a short latent period, which could exert its activity in a wide range of temperature and pH, is a promising candidate to be exploited in the treatment of multiple drug-resistant Klebsiella pneumonia.

Key words: Klebsiella pneumonia, bacteriophage, biological characteristics, genomics