上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (7): 753-.doi: 10.3969/j.issn.1674-8115.2018.07.006

• 论著·基础研究 • 上一篇    下一篇

宫内营养过剩对子代单核细胞及NK细胞免疫反应的影响

林慧 *，陈茜 *，刘欣梅，黄荷凤   

  1. 上海交通大学医学院附属中国福利会国际和平妇幼保健院，上海200030
  • 出版日期:2018-07-28 发布日期:2018-07-30
  • 通讯作者: 黄荷凤，电子信箱：huanghefg@hotmail.com。
  • 作者简介:林慧（1993—），女，博士生；电子信箱：linhui_angela@126.com。陈茜（1988—），女，博士后，博士；电子信箱：xichen06@hotmail.com。*为共同第一作者。
  • 基金资助:
    中国博士后科学基金面上资助项目（2016M601616）；上海市青年科技英才扬帆计划项目（17YF1420700）

Intrauterine high-nutrition induced immunoreaction abnormality in monocytes and nature killer cells in offspring

LIN Hui*, CHEN Xi*, LIU Xin-mei, HUANG He-feng   

  1. International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2018-07-28 Published:2018-07-30
  • Supported by:
    China Postdoctoral Science Fund,2016M601616; Shanghai Sailing Program, 17YF1420700

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摘要： 目的·探讨宫内营养过剩对子代单核细胞及自然杀伤（nature killer，NK）细胞炎症因子表达的影响。方法·收集巨大儿（n10）及正常体质量新生儿（n18）的脐血，通过磁珠分选获得单核细胞及NK细胞。在单核细胞中加入PBS（对照）或脂多糖（lipopolysaccharide，LPS）处理，NK细胞中加入PBS（对照）或佛波酯（phorbol myristate acetate，PMA）+离子霉素（ionomycin，ION）处理。Real-time PCR及ELISA检测对应炎症因子表达水平。结果· PBS对照组中，巨大儿与正常体质量新生儿脐血中单核细胞炎症因子表达水平差异无统计学意义。LPS刺激后，巨大儿组IL-1α、IL-1β、TNF-α、IL-10、CCL5的mRNA及蛋白表达水平显著低于正常组。在PBS阴性对照及PMA+ION刺激后，巨大儿组NK细胞中TNF-α的mRNA及蛋白表达水平均显著高于正常组，而IFN-γ的表达水平差异无统计学意义。结论·宫内营养过剩可导致子代脐血中单核细胞对LPS的免疫应答功能受损，而NK细胞产生TNF-α的能力增强。

关键词: 巨大儿, 脐血, 单核细胞, NK细胞, 炎症因子

Abstract:

Objective · To explore the effect of intrauterine high-nutrition on cytokine in umbilical cord blood-derived monocytes and nature killer (NK) cells. Methods · Samples of umbilical cord blood were collected macrosomia (n10) and infants with normal birthweight (n18). Monocytes and NK cells were obtainedmagnetic sorting. Purified monocytes were stimulatedlipopolysaccharide (LPS), while purified NK cells were stimulatedphorbol myristate acetate (PMA) and ionomycin (ION). The of cytokines was detectedreal-time PCR and ELISA. Results · There was no significant difference in cytokine in the monocytes treated with PBS between macrosomia and infants with normal birthweight. However, dampened responses following LPS stimulation were observed in macrosomia group, including the mRNA and protein of IL-1α, IL-1β, TNF-α, IL-10 and CCL5. Moreover, mRNA and protein of TNF-α were significantly higher in NK cells macrosomia group, while no significant difference in IFN-γ was found. Conclusion · Intrauterine high-nutrition might result in dampened response in monocytes following LPS stimulation and exaggerated of TNF-α in NK cells.

Key words: macrosomia, umbilical cord blood, monocyte, nature killer cell, cytokine

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